Mediators of non-adrenergic non-cholinergic inhibitory neurotransmission in porcine jejunum

N. M. Matsuda, S. M. Miller, L. Sha, G. Farrugia, J. H. Szurszewski

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

The purpose of this study was to determine the non-adrenergic non-cholinergic inhibitory neurotransmitter in pig jejunum. Intracellular electrical activity was recorded from circular smooth muscle cells. Inhibitory junction potentials (IJPs) evoked by electrical field stimulation were inhibited by tetrodoto xin (1 μmol L-1), ω-conotoxin GVIA (0.1 μmol L-1) tetrodotoxin, apamin (1 μmol L-1), 1-[6-((17β-3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl]-1H-pyrrole-2, 5-dione (U-73122; 10 μmol L-1) but not by Nω-nitro-L- arginine (L-NNA; 100 μmol L-1), haemoglobin (10 μmol L -1), 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ; 10 μmol L-1) or 9-(tetrahydio-2-furyl)adenine (SQ-22536; 10 μmol L -1). S-nitroso-N-acetylpenicillamine (SNAP) hyperpolarized the membrane potential. This was inhibited by ODQ (3 μmol L-1) and charybdotoxin (0.1 μmol L-1). Adenosine-5-triphosphate (ATP; 100 μmol L-1) and 2-methylthio ATP (2-MeS-ATP; 100 μmol L -1) did not hyperpolarize the membrane potential and 6-N-N-diethyl-β-γ-dibromomethylene-D-adenosine-5′-triphosphate (ARL67156-, 100 μmol L-1) did not modify IJPs. Carbon monoxide (CO; 10%) and tricarbonyl dichlororuthenium dimer ([Ru(CO3Cl 2)]2; 100 μmol L-1) hyperpolarized the membrane potential however zinc, copper and tin protoporphyrin IX (100 μmol L-1) did not alter IJPs. Vasoactive intestinal peptide (VIP) hyperpolarized the membrane potential but 4-Cl-D-Phe6-Leu 17-VIP (1 μmol L-1) did not modify IJPs. Pituitary adenylate cyclase activating peptide (PA-CAP)38 (0.5 μmol L-1) hyperpolarized the membrane potential. This was inhibited by apamin (1 μmol L-1) but not by tetrodotoxin (1 μmol L-1). Pituitary adenylate cyclase activating peptide6-38 (1 μmol L-1) inhibited IJPs. These data suggest that inhibitory neurotransmission in pig jejunum is mediated partly by PACAP.

Original languageEnglish (US)
Pages (from-to)605-612
Number of pages8
JournalNeurogastroenterology and Motility
Volume16
Issue number5
DOIs
StatePublished - Oct 2004

Keywords

  • Gastrointestinal smooth muscle
  • Inhibitory junction potential
  • Jejunum
  • Non-adrenergic non-cholinergic
  • Pig
  • Pituitary adenylate cyclase-activating polypeptide

ASJC Scopus subject areas

  • Physiology
  • Endocrine and Autonomic Systems
  • Gastroenterology

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