Mediation of serotonin-induced analgesia by the 5HT2 receptor in the pentobarbital anesthetized mouse model

William A. Banks, Abba J. Kastin, Terrence L. Trentman, H. Scott Haynes, Blake G. Johnson, Z. Harry Galina

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Serotonin can induce analgesia when injected directly into the brain, but analgesia after peripheral administration has been more difficult to show. The pentobarbital anesthetized mouse (PAM) model, developed to alleviate some of the problems involved in the measurement of tail flick latency, was used to assess the action of peripherally administered serotonin. Mice were anesthetized with about 65 mg/kg of sodium pentobarbital IP and their tail flick latencies measured while they were in stage III anesthesia. In these anesthetized mice, IP serotonin induced a significant analgesia that was much more robust than that found in awake mice. The analgesic effect was dose-dependent from 0.25 mg/kg to 10 mg/kg but was not blocked by the antiopiate naltrexone. Of several psychotropic agents tested, only amitriptyline, mianserin and trazodone had significant effects on analgesia in the PAM model. The analgesic effect of serotonin was reproduced by the 5HT2 agonist DOI and totally blocked by the 5HT2 antagonist NPP. These results show the utility of the PAM model in studying nonopiate analgesia and suggest that the analgesic action of serotonin is mediated primarily through the 5HT2 receptor.

Original languageEnglish (US)
Pages (from-to)887-891
Number of pages5
JournalBrain Research Bulletin
Volume21
Issue number6
DOIs
StatePublished - 1988
Externally publishedYes

Fingerprint

Pentobarbital
Analgesia
Serotonin
Analgesics
Tail
Trazodone
Mianserin
Naltrexone
Amitriptyline
Anesthesia
Brain

Keywords

  • 5HT receptor
  • Analgesia
  • Anesthesia
  • Antidepressants
  • Opiates
  • Serotonin
  • Tail flick

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Banks, W. A., Kastin, A. J., Trentman, T. L., Haynes, H. S., Johnson, B. G., & Galina, Z. H. (1988). Mediation of serotonin-induced analgesia by the 5HT2 receptor in the pentobarbital anesthetized mouse model. Brain Research Bulletin, 21(6), 887-891. https://doi.org/10.1016/0361-9230(88)90022-6

Mediation of serotonin-induced analgesia by the 5HT2 receptor in the pentobarbital anesthetized mouse model. / Banks, William A.; Kastin, Abba J.; Trentman, Terrence L.; Haynes, H. Scott; Johnson, Blake G.; Galina, Z. Harry.

In: Brain Research Bulletin, Vol. 21, No. 6, 1988, p. 887-891.

Research output: Contribution to journalArticle

Banks, William A. ; Kastin, Abba J. ; Trentman, Terrence L. ; Haynes, H. Scott ; Johnson, Blake G. ; Galina, Z. Harry. / Mediation of serotonin-induced analgesia by the 5HT2 receptor in the pentobarbital anesthetized mouse model. In: Brain Research Bulletin. 1988 ; Vol. 21, No. 6. pp. 887-891.
@article{8f1dc72d3da64b4085003b33e5e3e482,
title = "Mediation of serotonin-induced analgesia by the 5HT2 receptor in the pentobarbital anesthetized mouse model",
abstract = "Serotonin can induce analgesia when injected directly into the brain, but analgesia after peripheral administration has been more difficult to show. The pentobarbital anesthetized mouse (PAM) model, developed to alleviate some of the problems involved in the measurement of tail flick latency, was used to assess the action of peripherally administered serotonin. Mice were anesthetized with about 65 mg/kg of sodium pentobarbital IP and their tail flick latencies measured while they were in stage III anesthesia. In these anesthetized mice, IP serotonin induced a significant analgesia that was much more robust than that found in awake mice. The analgesic effect was dose-dependent from 0.25 mg/kg to 10 mg/kg but was not blocked by the antiopiate naltrexone. Of several psychotropic agents tested, only amitriptyline, mianserin and trazodone had significant effects on analgesia in the PAM model. The analgesic effect of serotonin was reproduced by the 5HT2 agonist DOI and totally blocked by the 5HT2 antagonist NPP. These results show the utility of the PAM model in studying nonopiate analgesia and suggest that the analgesic action of serotonin is mediated primarily through the 5HT2 receptor.",
keywords = "5HT receptor, Analgesia, Anesthesia, Antidepressants, Opiates, Serotonin, Tail flick",
author = "Banks, {William A.} and Kastin, {Abba J.} and Trentman, {Terrence L.} and Haynes, {H. Scott} and Johnson, {Blake G.} and Galina, {Z. Harry}",
year = "1988",
doi = "10.1016/0361-9230(88)90022-6",
language = "English (US)",
volume = "21",
pages = "887--891",
journal = "Brain Research Bulletin",
issn = "0361-9230",
publisher = "Elsevier Inc.",
number = "6",

}

TY - JOUR

T1 - Mediation of serotonin-induced analgesia by the 5HT2 receptor in the pentobarbital anesthetized mouse model

AU - Banks, William A.

AU - Kastin, Abba J.

AU - Trentman, Terrence L.

AU - Haynes, H. Scott

AU - Johnson, Blake G.

AU - Galina, Z. Harry

PY - 1988

Y1 - 1988

N2 - Serotonin can induce analgesia when injected directly into the brain, but analgesia after peripheral administration has been more difficult to show. The pentobarbital anesthetized mouse (PAM) model, developed to alleviate some of the problems involved in the measurement of tail flick latency, was used to assess the action of peripherally administered serotonin. Mice were anesthetized with about 65 mg/kg of sodium pentobarbital IP and their tail flick latencies measured while they were in stage III anesthesia. In these anesthetized mice, IP serotonin induced a significant analgesia that was much more robust than that found in awake mice. The analgesic effect was dose-dependent from 0.25 mg/kg to 10 mg/kg but was not blocked by the antiopiate naltrexone. Of several psychotropic agents tested, only amitriptyline, mianserin and trazodone had significant effects on analgesia in the PAM model. The analgesic effect of serotonin was reproduced by the 5HT2 agonist DOI and totally blocked by the 5HT2 antagonist NPP. These results show the utility of the PAM model in studying nonopiate analgesia and suggest that the analgesic action of serotonin is mediated primarily through the 5HT2 receptor.

AB - Serotonin can induce analgesia when injected directly into the brain, but analgesia after peripheral administration has been more difficult to show. The pentobarbital anesthetized mouse (PAM) model, developed to alleviate some of the problems involved in the measurement of tail flick latency, was used to assess the action of peripherally administered serotonin. Mice were anesthetized with about 65 mg/kg of sodium pentobarbital IP and their tail flick latencies measured while they were in stage III anesthesia. In these anesthetized mice, IP serotonin induced a significant analgesia that was much more robust than that found in awake mice. The analgesic effect was dose-dependent from 0.25 mg/kg to 10 mg/kg but was not blocked by the antiopiate naltrexone. Of several psychotropic agents tested, only amitriptyline, mianserin and trazodone had significant effects on analgesia in the PAM model. The analgesic effect of serotonin was reproduced by the 5HT2 agonist DOI and totally blocked by the 5HT2 antagonist NPP. These results show the utility of the PAM model in studying nonopiate analgesia and suggest that the analgesic action of serotonin is mediated primarily through the 5HT2 receptor.

KW - 5HT receptor

KW - Analgesia

KW - Anesthesia

KW - Antidepressants

KW - Opiates

KW - Serotonin

KW - Tail flick

UR - http://www.scopus.com/inward/record.url?scp=0024155543&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024155543&partnerID=8YFLogxK

U2 - 10.1016/0361-9230(88)90022-6

DO - 10.1016/0361-9230(88)90022-6

M3 - Article

VL - 21

SP - 887

EP - 891

JO - Brain Research Bulletin

JF - Brain Research Bulletin

SN - 0361-9230

IS - 6

ER -