Abstract
Changes in troponin C (TnC) conformation upon Ca2+ binding forms the basis for regulatory and structural functions of TnC molecules. In the present study, Ca2+-induced conformational changes in TnC were observed by mechanical measurements. TnC films were prepared by drying or electrospraying TnC solutions, cross-linked with glutaraldehyde, and isometric tension and stiffness measured as a function of pCa. An increase in Ca2+ from a pCa of 9 to 4 induced large-scale mechanical changes in the TnC films causing several percent shrinkage of the unloaded films. This shrinkage could be partially assigned to Ca2+ binding to the Ca2+/Mg2+ sites of TnC.
Original language | English (US) |
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Pages (from-to) | 107-110 |
Number of pages | 4 |
Journal | FEBS Letters |
Volume | 524 |
Issue number | 1-3 |
DOIs | |
State | Published - Jul 31 2002 |
Keywords
- Calcium
- Calcium-binding protein
- Conformational change
- Cross-linking
- Protein film
- Troponin C
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology