Mechano-chemical effects of Ca2+ in cross-linked troponin-C films

Anna E. Bukatina, Victor N. Morozov, Nikolai B. Gusev, Gary C. Sieck

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Changes in troponin C (TnC) conformation upon Ca2+ binding forms the basis for regulatory and structural functions of TnC molecules. In the present study, Ca2+-induced conformational changes in TnC were observed by mechanical measurements. TnC films were prepared by drying or electrospraying TnC solutions, cross-linked with glutaraldehyde, and isometric tension and stiffness measured as a function of pCa. An increase in Ca2+ from a pCa of 9 to 4 induced large-scale mechanical changes in the TnC films causing several percent shrinkage of the unloaded films. This shrinkage could be partially assigned to Ca2+ binding to the Ca2+/Mg2+ sites of TnC.

Original languageEnglish (US)
Pages (from-to)107-110
Number of pages4
JournalFEBS Letters
Volume524
Issue number1-3
DOIs
StatePublished - Jul 31 2002

Keywords

  • Calcium
  • Calcium-binding protein
  • Conformational change
  • Cross-linking
  • Protein film
  • Troponin C

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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    Bukatina, A. E., Morozov, V. N., Gusev, N. B., & Sieck, G. C. (2002). Mechano-chemical effects of Ca2+ in cross-linked troponin-C films. FEBS Letters, 524(1-3), 107-110. https://doi.org/10.1016/S0014-5793(02)03013-2