Abstract
Aim: This study aims to determine mechanisms of action of the gasotransmitter hydrogen sulfide (H2S) on contractile activity in longitudinal muscle of rat ileum. Methods: Ileal longitudinal muscle strips were prepared to measure isometric contractions. Effects of sodium hydrosulfide (NaHS), a donor of H2S, were evaluated on spontaneous contractile activity and after enhanced contractile activity with bethanechol. l-cysteine was evaluated as a potential endogenous donor of H2S. We evaluated involvement of extrinsic nerves, enteric nervous system, visceral afferent nerves, nitric oxide, and KATP+ channel and KCa+ channel activity on the action of H2S using non-adrenergic/non-cholinergic conditions, tetrodotoxin, capsaicin, l-NG-nitro arginine (l-NNA), glibenclamide, and apamin, respectively, as well as electrical field stimulation. Result: NaHS dose-dependently and reversibly inhibited spontaneous and bethanechol-stimulated contractile activity (p < 0.05). l-cysteine had no inhibitory effect. Non-adrenergic/non-cholinergic conditions, tetrodotoxin, capsaicin, l-NNA, glibenclamide, or apamin had no major effect on total contractile activity by NaHS, although both tetrodotoxin and apamin decreased the frequency of bethanechol-enhanced contractile activity (p < 0.05). We could not demonstrate H2S release by electrical field stimulation but did show that inhibition of cystathionine β synthase, an endogenous source of H2S, augmented the inhibitory effect of low-frequency electrical field stimulation. Conclusion: H2S inhibits contractile activity of ileal longitudinal muscle dose-dependently but not through pathways mediated by the extrinsic or enteric nervous system, visceral afferent nerves, nitric oxide, KATP+ channels, or KCa+ channels.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 12-22 |
| Number of pages | 11 |
| Journal | Journal of Gastrointestinal Surgery |
| Volume | 15 |
| Issue number | 1 |
| DOIs | |
| State | Published - 2011 |
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Keywords
- Contractile activity
- Gasotransmitter
- Hydrogen sulfide
- Ileum longitudinal smooth muscle
- Intestinal motility
- Longitudinal muscle
- Motility
- Physiology
ASJC Scopus subject areas
- Surgery
- Gastroenterology
Cite this
Mechanisms of Action of the Gasotransmitter Hydrogen Sulfide in Modulating Contractile Activity of Longitudinal Muscle of Rat Ileum. / Nagao, Munenori; Linden, David R; Duenes, Judith A.; Sarr, Michael G.
In: Journal of Gastrointestinal Surgery, Vol. 15, No. 1, 2011, p. 12-22.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Mechanisms of Action of the Gasotransmitter Hydrogen Sulfide in Modulating Contractile Activity of Longitudinal Muscle of Rat Ileum
AU - Nagao, Munenori
AU - Linden, David R
AU - Duenes, Judith A.
AU - Sarr, Michael G.
PY - 2011
Y1 - 2011
N2 - Aim: This study aims to determine mechanisms of action of the gasotransmitter hydrogen sulfide (H2S) on contractile activity in longitudinal muscle of rat ileum. Methods: Ileal longitudinal muscle strips were prepared to measure isometric contractions. Effects of sodium hydrosulfide (NaHS), a donor of H2S, were evaluated on spontaneous contractile activity and after enhanced contractile activity with bethanechol. l-cysteine was evaluated as a potential endogenous donor of H2S. We evaluated involvement of extrinsic nerves, enteric nervous system, visceral afferent nerves, nitric oxide, and KATP+ channel and KCa+ channel activity on the action of H2S using non-adrenergic/non-cholinergic conditions, tetrodotoxin, capsaicin, l-NG-nitro arginine (l-NNA), glibenclamide, and apamin, respectively, as well as electrical field stimulation. Result: NaHS dose-dependently and reversibly inhibited spontaneous and bethanechol-stimulated contractile activity (p < 0.05). l-cysteine had no inhibitory effect. Non-adrenergic/non-cholinergic conditions, tetrodotoxin, capsaicin, l-NNA, glibenclamide, or apamin had no major effect on total contractile activity by NaHS, although both tetrodotoxin and apamin decreased the frequency of bethanechol-enhanced contractile activity (p < 0.05). We could not demonstrate H2S release by electrical field stimulation but did show that inhibition of cystathionine β synthase, an endogenous source of H2S, augmented the inhibitory effect of low-frequency electrical field stimulation. Conclusion: H2S inhibits contractile activity of ileal longitudinal muscle dose-dependently but not through pathways mediated by the extrinsic or enteric nervous system, visceral afferent nerves, nitric oxide, KATP+ channels, or KCa+ channels.
AB - Aim: This study aims to determine mechanisms of action of the gasotransmitter hydrogen sulfide (H2S) on contractile activity in longitudinal muscle of rat ileum. Methods: Ileal longitudinal muscle strips were prepared to measure isometric contractions. Effects of sodium hydrosulfide (NaHS), a donor of H2S, were evaluated on spontaneous contractile activity and after enhanced contractile activity with bethanechol. l-cysteine was evaluated as a potential endogenous donor of H2S. We evaluated involvement of extrinsic nerves, enteric nervous system, visceral afferent nerves, nitric oxide, and KATP+ channel and KCa+ channel activity on the action of H2S using non-adrenergic/non-cholinergic conditions, tetrodotoxin, capsaicin, l-NG-nitro arginine (l-NNA), glibenclamide, and apamin, respectively, as well as electrical field stimulation. Result: NaHS dose-dependently and reversibly inhibited spontaneous and bethanechol-stimulated contractile activity (p < 0.05). l-cysteine had no inhibitory effect. Non-adrenergic/non-cholinergic conditions, tetrodotoxin, capsaicin, l-NNA, glibenclamide, or apamin had no major effect on total contractile activity by NaHS, although both tetrodotoxin and apamin decreased the frequency of bethanechol-enhanced contractile activity (p < 0.05). We could not demonstrate H2S release by electrical field stimulation but did show that inhibition of cystathionine β synthase, an endogenous source of H2S, augmented the inhibitory effect of low-frequency electrical field stimulation. Conclusion: H2S inhibits contractile activity of ileal longitudinal muscle dose-dependently but not through pathways mediated by the extrinsic or enteric nervous system, visceral afferent nerves, nitric oxide, KATP+ channels, or KCa+ channels.
KW - Contractile activity
KW - Gasotransmitter
KW - Hydrogen sulfide
KW - Ileum longitudinal smooth muscle
KW - Intestinal motility
KW - Longitudinal muscle
KW - Motility
KW - Physiology
UR - http://www.scopus.com/inward/record.url?scp=78751591549&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78751591549&partnerID=8YFLogxK
U2 - 10.1007/s11605-010-1306-8
DO - 10.1007/s11605-010-1306-8
M3 - Article
C2 - 21082276
AN - SCOPUS:78751591549
VL - 15
SP - 12
EP - 22
JO - Journal of Gastrointestinal Surgery
JF - Journal of Gastrointestinal Surgery
SN - 1091-255X
IS - 1
ER -