Mechanical properties of cultured human airway smooth muscle cells from 0.05 to 0.4 Hz

Geoffrey N. Maksym, Ben Fabry, James P. Butler, Daniel Navajas, Daniel J. Tschumperlin, Johanne D. Laporte, Jeffrey J. Fredberg

Research output: Contribution to journalArticle

142 Scopus citations

Abstract

We investigated the rheological properties of living human airway smooth muscle cells in culture and monitored the changes in rheological properties induced by exogenous stimuli. We oscillated small magnetic microbeads bound specifically to integrin receptors and computed the storage modulus (g') and loss modulus (g') from the applied torque and the resulting rotational motion of the beads as determined from their remanent magnetic field. Under baseline conditions, G' increased weakly with frequency, whereas G' was independent of the frequency. The cell was predominantly elastic, with the ratio of G' to G' (defined as η) being κ0.35 at all frequencies. G' and G' increased together after contractile activation and decreased together after deactivation, whereas η remained unaltered in each case. Thus elastic and dissipative stresses were coupled during changes in contractile activation. G' and G' decreased with disruption of the actin fibers by cytochalasin D, but η increased. These results imply that the mechanisms for frictional energy loss and elastic energy storage in the living cell are coupled and reside within the cytoskeleton.

Original languageEnglish (US)
Pages (from-to)1619-1632
Number of pages14
JournalJournal of applied physiology
Volume89
Issue number4
DOIs
StatePublished - 2000

Keywords

  • Contraction
  • Cytoskeleton
  • Magnetic twisting cytometry
  • Storage modulus
  • Structural damping
  • Viscoelasticity

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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