Mechanical properties of alveolar epithelial cells in culture

Jorge C. Berrios, Mark A. Schroeder, Rolf D. Hubmayr

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

With the use of magnetic twisting cytometry, we characterized the mechanical properties of rat type II alveolar epithelial (ATII) cells in primary culture and examined whether the cells' state of differentiation and the application of deforming stresses influence their resistance to shape change. Cells were harvested from rat lungs as previously described (Dobbs LG. Am J Physiol Lung Cell Mol Physiol 258: L134-L147, 1990) and plated at a density of 1 × 10 6 cells/cm 2 in fibronectin-coated 96 Remova wells, and their mechanical properties were measured 2-9 days later. We show 1) that ATII cells form much stronger bonds with RGD-coated beads than they do with albumin- or acetylated low-density lipoprotein-coated beads, 2) that RGD-mediated bonds seemingly "mature" during the first 60 min of bead contact, 3) that the apparent stiffness of ATII cells increases with days in culture, 4) that stiffness falls when the RGD-coated beads are intermittently oscillated at 0.3 Hz, and 5) that this fall cannot be attributed to exocytosis-related remodeling of the subcortical cytoskeleton. Although the mechanisms of force transfer between basement membrane, cytoskeleton, and plasma membrane of ATII cells remain to be resolved, such analyses undoubtedly require definition of the cell's mechanical properties. To our knowledge, the results presented here provide the first data on this topic.

Original languageEnglish (US)
Pages (from-to)65-73
Number of pages9
JournalJournal of Applied Physiology
Volume91
Issue number1
StatePublished - 2001

Fingerprint

Alveolar Epithelial Cells
Cell Culture Techniques
alpha-allylaminopropion-o-toluidide
Cytoskeleton
Lung
Exocytosis
Fibronectins
Basement Membrane
Cell Differentiation
Albumins
Cell Membrane

Keywords

  • Cell adhesion
  • Cytoskeleton
  • Lung injury
  • Magnetic twisting cytometry
  • Mechanical stress

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Berrios, J. C., Schroeder, M. A., & Hubmayr, R. D. (2001). Mechanical properties of alveolar epithelial cells in culture. Journal of Applied Physiology, 91(1), 65-73.

Mechanical properties of alveolar epithelial cells in culture. / Berrios, Jorge C.; Schroeder, Mark A.; Hubmayr, Rolf D.

In: Journal of Applied Physiology, Vol. 91, No. 1, 2001, p. 65-73.

Research output: Contribution to journalArticle

Berrios, JC, Schroeder, MA & Hubmayr, RD 2001, 'Mechanical properties of alveolar epithelial cells in culture', Journal of Applied Physiology, vol. 91, no. 1, pp. 65-73.
Berrios, Jorge C. ; Schroeder, Mark A. ; Hubmayr, Rolf D. / Mechanical properties of alveolar epithelial cells in culture. In: Journal of Applied Physiology. 2001 ; Vol. 91, No. 1. pp. 65-73.
@article{a931a2b6b4424deeb8a8de197be27761,
title = "Mechanical properties of alveolar epithelial cells in culture",
abstract = "With the use of magnetic twisting cytometry, we characterized the mechanical properties of rat type II alveolar epithelial (ATII) cells in primary culture and examined whether the cells' state of differentiation and the application of deforming stresses influence their resistance to shape change. Cells were harvested from rat lungs as previously described (Dobbs LG. Am J Physiol Lung Cell Mol Physiol 258: L134-L147, 1990) and plated at a density of 1 × 10 6 cells/cm 2 in fibronectin-coated 96 Remova wells, and their mechanical properties were measured 2-9 days later. We show 1) that ATII cells form much stronger bonds with RGD-coated beads than they do with albumin- or acetylated low-density lipoprotein-coated beads, 2) that RGD-mediated bonds seemingly {"}mature{"} during the first 60 min of bead contact, 3) that the apparent stiffness of ATII cells increases with days in culture, 4) that stiffness falls when the RGD-coated beads are intermittently oscillated at 0.3 Hz, and 5) that this fall cannot be attributed to exocytosis-related remodeling of the subcortical cytoskeleton. Although the mechanisms of force transfer between basement membrane, cytoskeleton, and plasma membrane of ATII cells remain to be resolved, such analyses undoubtedly require definition of the cell's mechanical properties. To our knowledge, the results presented here provide the first data on this topic.",
keywords = "Cell adhesion, Cytoskeleton, Lung injury, Magnetic twisting cytometry, Mechanical stress",
author = "Berrios, {Jorge C.} and Schroeder, {Mark A.} and Hubmayr, {Rolf D.}",
year = "2001",
language = "English (US)",
volume = "91",
pages = "65--73",
journal = "Journal of Applied Physiology",
issn = "8750-7587",
publisher = "American Physiological Society",
number = "1",

}

TY - JOUR

T1 - Mechanical properties of alveolar epithelial cells in culture

AU - Berrios, Jorge C.

AU - Schroeder, Mark A.

AU - Hubmayr, Rolf D.

PY - 2001

Y1 - 2001

N2 - With the use of magnetic twisting cytometry, we characterized the mechanical properties of rat type II alveolar epithelial (ATII) cells in primary culture and examined whether the cells' state of differentiation and the application of deforming stresses influence their resistance to shape change. Cells were harvested from rat lungs as previously described (Dobbs LG. Am J Physiol Lung Cell Mol Physiol 258: L134-L147, 1990) and plated at a density of 1 × 10 6 cells/cm 2 in fibronectin-coated 96 Remova wells, and their mechanical properties were measured 2-9 days later. We show 1) that ATII cells form much stronger bonds with RGD-coated beads than they do with albumin- or acetylated low-density lipoprotein-coated beads, 2) that RGD-mediated bonds seemingly "mature" during the first 60 min of bead contact, 3) that the apparent stiffness of ATII cells increases with days in culture, 4) that stiffness falls when the RGD-coated beads are intermittently oscillated at 0.3 Hz, and 5) that this fall cannot be attributed to exocytosis-related remodeling of the subcortical cytoskeleton. Although the mechanisms of force transfer between basement membrane, cytoskeleton, and plasma membrane of ATII cells remain to be resolved, such analyses undoubtedly require definition of the cell's mechanical properties. To our knowledge, the results presented here provide the first data on this topic.

AB - With the use of magnetic twisting cytometry, we characterized the mechanical properties of rat type II alveolar epithelial (ATII) cells in primary culture and examined whether the cells' state of differentiation and the application of deforming stresses influence their resistance to shape change. Cells were harvested from rat lungs as previously described (Dobbs LG. Am J Physiol Lung Cell Mol Physiol 258: L134-L147, 1990) and plated at a density of 1 × 10 6 cells/cm 2 in fibronectin-coated 96 Remova wells, and their mechanical properties were measured 2-9 days later. We show 1) that ATII cells form much stronger bonds with RGD-coated beads than they do with albumin- or acetylated low-density lipoprotein-coated beads, 2) that RGD-mediated bonds seemingly "mature" during the first 60 min of bead contact, 3) that the apparent stiffness of ATII cells increases with days in culture, 4) that stiffness falls when the RGD-coated beads are intermittently oscillated at 0.3 Hz, and 5) that this fall cannot be attributed to exocytosis-related remodeling of the subcortical cytoskeleton. Although the mechanisms of force transfer between basement membrane, cytoskeleton, and plasma membrane of ATII cells remain to be resolved, such analyses undoubtedly require definition of the cell's mechanical properties. To our knowledge, the results presented here provide the first data on this topic.

KW - Cell adhesion

KW - Cytoskeleton

KW - Lung injury

KW - Magnetic twisting cytometry

KW - Mechanical stress

UR - http://www.scopus.com/inward/record.url?scp=0034972604&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034972604&partnerID=8YFLogxK

M3 - Article

C2 - 11408414

AN - SCOPUS:0034972604

VL - 91

SP - 65

EP - 73

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

SN - 8750-7587

IS - 1

ER -