Measuring reactive oxygen species in senescent cells

Joao Passos, Satomi Miwa, Thomas Von Zglinicki

Research output: Chapter in Book/Report/Conference proceedingChapter

10 Citations (Scopus)

Abstract

Countless studies have implicated reactive oxygen species (ROS) and mitochondrial dysfunction in the ageing process. During cellular senescence, the ultimate and irreversible loss of replicative capacity of somatic cells grown in culture, several studies have reported increased levels of ROS associated with mitochondrial dysfunction and metabolic inefficiency. Moreover, studies have revealed that interventions modulating intracellular ROS can impact on the replicative lifespan of cultured cells, suggesting that ROS play a central role in the process. In this chapter, we present several protocols used for detection of (intra- and extracellular) ROS in live cells.

Original languageEnglish (US)
Title of host publicationCell Senescence
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages253-263
Number of pages11
ISBN (Print)9781627032384
DOIs
StatePublished - Jan 1 2013
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume965
ISSN (Print)1064-3745

Fingerprint

Reactive Oxygen Species
Cell Aging
Cultured Cells

Keywords

  • Amplex® Red
  • DHE
  • DHR123
  • MitoSOX™
  • ROS
  • ROS indicator dyes
  • Senescence

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

Cite this

Passos, J., Miwa, S., & Von Zglinicki, T. (2013). Measuring reactive oxygen species in senescent cells. In Cell Senescence: Methods and Protocols (pp. 253-263). (Methods in Molecular Biology; Vol. 965). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-239-1_17

Measuring reactive oxygen species in senescent cells. / Passos, Joao; Miwa, Satomi; Von Zglinicki, Thomas.

Cell Senescence: Methods and Protocols. Humana Press Inc., 2013. p. 253-263 (Methods in Molecular Biology; Vol. 965).

Research output: Chapter in Book/Report/Conference proceedingChapter

Passos, J, Miwa, S & Von Zglinicki, T 2013, Measuring reactive oxygen species in senescent cells. in Cell Senescence: Methods and Protocols. Methods in Molecular Biology, vol. 965, Humana Press Inc., pp. 253-263. https://doi.org/10.1007/978-1-62703-239-1_17
Passos J, Miwa S, Von Zglinicki T. Measuring reactive oxygen species in senescent cells. In Cell Senescence: Methods and Protocols. Humana Press Inc. 2013. p. 253-263. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-239-1_17
Passos, Joao ; Miwa, Satomi ; Von Zglinicki, Thomas. / Measuring reactive oxygen species in senescent cells. Cell Senescence: Methods and Protocols. Humana Press Inc., 2013. pp. 253-263 (Methods in Molecular Biology).
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