Measuring luteinising hormone pulsatility with a robotic aptamer-enabled electrochemical reader

Shaolin Liang, Andrew B. Kinghorn, Margaritis Voliotis, Julia K. Prague, Johannes D Veldhuis, Krasimira Tsaneva-Atanasova, Craig A. McArdle, Raymond H.W. Li, Anthony E.G. Cass, Waljit S. Dhillo, Julian A. Tanner

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Normal reproductive functioning is critically dependent on pulsatile secretion of luteinising hormone (LH). Assessment of LH pulsatility is important for the clinical diagnosis of reproductive disorders, but current methods are hampered by frequent blood sampling coupled to expensive serial immunochemical analysis. Here, we report the development and application of a Robotic APTamer-enabled Electrochemical Reader (RAPTER) electrochemical analysis system to determine LH pulsatility. Through selective evolution of ligands by exponential enrichment (SELEX), we identify DNA aptamers that bind specifically to LH and not to related hormones. The aptamers are integrated into electrochemical aptamer-based (E-AB) sensors on a robotic platform. E-AB enables rapid, sensitive and repeatable determination of LH concentration profiles. Bayesian Spectrum Analysis is applied to determine LH pulsatility in three distinct patient cohorts. This technology has the potential to transform the clinical care of patients with reproductive disorders and could be developed to allow real-time in vivo hormone monitoring.

Original languageEnglish (US)
Article number852
JournalNature communications
Volume10
Issue number1
DOIs
StatePublished - Dec 1 2019

Fingerprint

hormones
Robotics
readers
Luteinizing Hormone
robotics
Hormones
Nucleotide Aptamers
Bayes Theorem
disorders
Spectrum analysis
secretions
Spectrum Analysis
Patient Care
systems analysis
Blood
Sampling
Ligands
blood
Technology
spectrum analysis

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Liang, S., Kinghorn, A. B., Voliotis, M., Prague, J. K., Veldhuis, J. D., Tsaneva-Atanasova, K., ... Tanner, J. A. (2019). Measuring luteinising hormone pulsatility with a robotic aptamer-enabled electrochemical reader. Nature communications, 10(1), [852]. https://doi.org/10.1038/s41467-019-08799-6

Measuring luteinising hormone pulsatility with a robotic aptamer-enabled electrochemical reader. / Liang, Shaolin; Kinghorn, Andrew B.; Voliotis, Margaritis; Prague, Julia K.; Veldhuis, Johannes D; Tsaneva-Atanasova, Krasimira; McArdle, Craig A.; Li, Raymond H.W.; Cass, Anthony E.G.; Dhillo, Waljit S.; Tanner, Julian A.

In: Nature communications, Vol. 10, No. 1, 852, 01.12.2019.

Research output: Contribution to journalArticle

Liang, S, Kinghorn, AB, Voliotis, M, Prague, JK, Veldhuis, JD, Tsaneva-Atanasova, K, McArdle, CA, Li, RHW, Cass, AEG, Dhillo, WS & Tanner, JA 2019, 'Measuring luteinising hormone pulsatility with a robotic aptamer-enabled electrochemical reader', Nature communications, vol. 10, no. 1, 852. https://doi.org/10.1038/s41467-019-08799-6
Liang S, Kinghorn AB, Voliotis M, Prague JK, Veldhuis JD, Tsaneva-Atanasova K et al. Measuring luteinising hormone pulsatility with a robotic aptamer-enabled electrochemical reader. Nature communications. 2019 Dec 1;10(1). 852. https://doi.org/10.1038/s41467-019-08799-6
Liang, Shaolin ; Kinghorn, Andrew B. ; Voliotis, Margaritis ; Prague, Julia K. ; Veldhuis, Johannes D ; Tsaneva-Atanasova, Krasimira ; McArdle, Craig A. ; Li, Raymond H.W. ; Cass, Anthony E.G. ; Dhillo, Waljit S. ; Tanner, Julian A. / Measuring luteinising hormone pulsatility with a robotic aptamer-enabled electrochemical reader. In: Nature communications. 2019 ; Vol. 10, No. 1.
@article{aa4ecc61e11244acb54906e328cf965d,
title = "Measuring luteinising hormone pulsatility with a robotic aptamer-enabled electrochemical reader",
abstract = "Normal reproductive functioning is critically dependent on pulsatile secretion of luteinising hormone (LH). Assessment of LH pulsatility is important for the clinical diagnosis of reproductive disorders, but current methods are hampered by frequent blood sampling coupled to expensive serial immunochemical analysis. Here, we report the development and application of a Robotic APTamer-enabled Electrochemical Reader (RAPTER) electrochemical analysis system to determine LH pulsatility. Through selective evolution of ligands by exponential enrichment (SELEX), we identify DNA aptamers that bind specifically to LH and not to related hormones. The aptamers are integrated into electrochemical aptamer-based (E-AB) sensors on a robotic platform. E-AB enables rapid, sensitive and repeatable determination of LH concentration profiles. Bayesian Spectrum Analysis is applied to determine LH pulsatility in three distinct patient cohorts. This technology has the potential to transform the clinical care of patients with reproductive disorders and could be developed to allow real-time in vivo hormone monitoring.",
author = "Shaolin Liang and Kinghorn, {Andrew B.} and Margaritis Voliotis and Prague, {Julia K.} and Veldhuis, {Johannes D} and Krasimira Tsaneva-Atanasova and McArdle, {Craig A.} and Li, {Raymond H.W.} and Cass, {Anthony E.G.} and Dhillo, {Waljit S.} and Tanner, {Julian A.}",
year = "2019",
month = "12",
day = "1",
doi = "10.1038/s41467-019-08799-6",
language = "English (US)",
volume = "10",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - Measuring luteinising hormone pulsatility with a robotic aptamer-enabled electrochemical reader

AU - Liang, Shaolin

AU - Kinghorn, Andrew B.

AU - Voliotis, Margaritis

AU - Prague, Julia K.

AU - Veldhuis, Johannes D

AU - Tsaneva-Atanasova, Krasimira

AU - McArdle, Craig A.

AU - Li, Raymond H.W.

AU - Cass, Anthony E.G.

AU - Dhillo, Waljit S.

AU - Tanner, Julian A.

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Normal reproductive functioning is critically dependent on pulsatile secretion of luteinising hormone (LH). Assessment of LH pulsatility is important for the clinical diagnosis of reproductive disorders, but current methods are hampered by frequent blood sampling coupled to expensive serial immunochemical analysis. Here, we report the development and application of a Robotic APTamer-enabled Electrochemical Reader (RAPTER) electrochemical analysis system to determine LH pulsatility. Through selective evolution of ligands by exponential enrichment (SELEX), we identify DNA aptamers that bind specifically to LH and not to related hormones. The aptamers are integrated into electrochemical aptamer-based (E-AB) sensors on a robotic platform. E-AB enables rapid, sensitive and repeatable determination of LH concentration profiles. Bayesian Spectrum Analysis is applied to determine LH pulsatility in three distinct patient cohorts. This technology has the potential to transform the clinical care of patients with reproductive disorders and could be developed to allow real-time in vivo hormone monitoring.

AB - Normal reproductive functioning is critically dependent on pulsatile secretion of luteinising hormone (LH). Assessment of LH pulsatility is important for the clinical diagnosis of reproductive disorders, but current methods are hampered by frequent blood sampling coupled to expensive serial immunochemical analysis. Here, we report the development and application of a Robotic APTamer-enabled Electrochemical Reader (RAPTER) electrochemical analysis system to determine LH pulsatility. Through selective evolution of ligands by exponential enrichment (SELEX), we identify DNA aptamers that bind specifically to LH and not to related hormones. The aptamers are integrated into electrochemical aptamer-based (E-AB) sensors on a robotic platform. E-AB enables rapid, sensitive and repeatable determination of LH concentration profiles. Bayesian Spectrum Analysis is applied to determine LH pulsatility in three distinct patient cohorts. This technology has the potential to transform the clinical care of patients with reproductive disorders and could be developed to allow real-time in vivo hormone monitoring.

UR - http://www.scopus.com/inward/record.url?scp=85061789267&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85061789267&partnerID=8YFLogxK

U2 - 10.1038/s41467-019-08799-6

DO - 10.1038/s41467-019-08799-6

M3 - Article

C2 - 30787284

AN - SCOPUS:85061789267

VL - 10

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

M1 - 852

ER -