Measuring binding interactions of neurite-extension promoting antibodies to supported lipid membranes using a multichannel microfluidic plasmonic nanohole array biosensor

L. Jordan, X. Xu, N. J. Wittenberg, A. E. Warrington, A. Denic, B. Wootla, D. Yoo, J. Watzlawik, M. Rodriguez, S. H. Oh

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

We present a plasmonic nanohole array biosensor capable of measuring spectral changes due to molecular binding interactions across multiple channels with an imaging spectrometer [1-3]. Lipid bilayers doped with gangliosides were formed on the sensor surface and an antibody, rHIgM12, was injected over these membranes and binding was observed. This antibody has previously been shown to promote neurite-extension in neurons [4-6] and thus may have therapeutic benefits. The hypothesized receptors are gangliosides, GD1a and GT1b. Specificity to these gangliosides was observed, and the dissociation constant KD to GT1b was calculated to be 12.9 ?} 7.2 nM.

Original languageEnglish (US)
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages215-217
Number of pages3
ISBN (Print)9781632666246
StatePublished - Jan 1 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: Oct 27 2013Oct 31 2013

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume1

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period10/27/1310/31/13

Keywords

  • Antibodies
  • Multichannel
  • Plasmonic
  • Supported lipid bilayer

ASJC Scopus subject areas

  • Bioengineering

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  • Cite this

    Jordan, L., Xu, X., Wittenberg, N. J., Warrington, A. E., Denic, A., Wootla, B., Yoo, D., Watzlawik, J., Rodriguez, M., & Oh, S. H. (2013). Measuring binding interactions of neurite-extension promoting antibodies to supported lipid membranes using a multichannel microfluidic plasmonic nanohole array biosensor. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 215-217). (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 1). Chemical and Biological Microsystems Society.