Measurements of pertinent concentrations of oxygen in vivo

H. M. Swartz, S. Boyer, P. Gast, James Glockner, H. Hu, K. J. Liu, M. Moussavi, S. W. Norby, N. Vahidi, T. Walczak, M. Wu, R. B. Clarkson

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Abstract

A new method able to measure the concentration of oxygen in complex biological systems, including in vivo, has been developed using low-frequency EPR and newly characterized free radicals that are very sensitive to the concentration of oxygen. The free radicals (fusinite and lithium phthalocyanine) are very stable in tissues (for at least 150 days), apparently nontoxic, and can reflect oxygen concentrations that are less than the K(m) of cytochrome oxidase (0.1 μM or lower). Their biological stability is indicated by the fact that repeated measurements with fusinite of the concentration of oxygen in skeletal muscle have been made in the same animal for more than 150 days without any change in sensitivity or signs of toxicity.

Original languageEnglish (US)
Pages (from-to)333-339
Number of pages7
JournalMagnetic Resonance in Medicine
Volume20
Issue number2
StatePublished - 1991
Externally publishedYes

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Oxygen
Free Radicals
Electron Transport Complex IV
Skeletal Muscle
fusinite
lithium phthalocyanine

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Swartz, H. M., Boyer, S., Gast, P., Glockner, J., Hu, H., Liu, K. J., ... Clarkson, R. B. (1991). Measurements of pertinent concentrations of oxygen in vivo. Magnetic Resonance in Medicine, 20(2), 333-339.

Measurements of pertinent concentrations of oxygen in vivo. / Swartz, H. M.; Boyer, S.; Gast, P.; Glockner, James; Hu, H.; Liu, K. J.; Moussavi, M.; Norby, S. W.; Vahidi, N.; Walczak, T.; Wu, M.; Clarkson, R. B.

In: Magnetic Resonance in Medicine, Vol. 20, No. 2, 1991, p. 333-339.

Research output: Contribution to journalArticle

Swartz, HM, Boyer, S, Gast, P, Glockner, J, Hu, H, Liu, KJ, Moussavi, M, Norby, SW, Vahidi, N, Walczak, T, Wu, M & Clarkson, RB 1991, 'Measurements of pertinent concentrations of oxygen in vivo', Magnetic Resonance in Medicine, vol. 20, no. 2, pp. 333-339.
Swartz HM, Boyer S, Gast P, Glockner J, Hu H, Liu KJ et al. Measurements of pertinent concentrations of oxygen in vivo. Magnetic Resonance in Medicine. 1991;20(2):333-339.
Swartz, H. M. ; Boyer, S. ; Gast, P. ; Glockner, James ; Hu, H. ; Liu, K. J. ; Moussavi, M. ; Norby, S. W. ; Vahidi, N. ; Walczak, T. ; Wu, M. ; Clarkson, R. B. / Measurements of pertinent concentrations of oxygen in vivo. In: Magnetic Resonance in Medicine. 1991 ; Vol. 20, No. 2. pp. 333-339.
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