Measurements of Fluorescence Lifetimes by Use of a Hybrid Time-Correlated and Multifrequency Phase Fluorometer

J. Hedstrom Sedarous, S. Sedarous Sedarous, F. G. Prendergast

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Measurements of homogeneous and heterogeneous fluorescence intensity decays using a hybrid time-correlated single photon counting/multifrequency phase fluorometer are reported. A trio of fluorophores exhibiting a range of decay profiles was selected. p-Terphenyl, l,4-bis[2-(4-methyl-5-phenyloxazolyl)]benzene [(Me)2POPOP], and p-bis[2-(5-phenyloxazolyl)]benzene (POPOP), commonly used reference fluorophores, were analyzed initially; their emissions were characterized by monoexponential decay functions. Additionally, emissions from two single tryptophan proteins with different decay profiles were measured. Scorpion neurotoxin variant 3 required three exponentials to fit the emission decay properly (average lifetime ~500 ps). At pH 5.5, the fluorescence emission of ribonuclease T1 showed a monoexponential decay with a measured lifetime of ~4.0 ns. Thus, in each case, the results from both measurements were consistent between the two detection systems, confirming the view that the two approaches for measuring fluorescence lifetimes are equivalent.

Original languageEnglish (US)
Pages (from-to)6203-6208
Number of pages6
JournalBiochemistry
Volume27
Issue number17
DOIs
StatePublished - Aug 1 1988

ASJC Scopus subject areas

  • Biochemistry

Fingerprint Dive into the research topics of 'Measurements of Fluorescence Lifetimes by Use of a Hybrid Time-Correlated and Multifrequency Phase Fluorometer'. Together they form a unique fingerprint.

  • Cite this