Measurement of plasma glycerol specific activity by high performance liquid chromatography to determine glycerol flux

Robert L. Judd, Rita Nelson, Samuel Klein, Michael D. Jensen, John M. Miles

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Previous methods for measuring plasma glycerol specific activity (SA) are suboptimal, making the determination of glycerol kinetics in vivo with radiotracers difficult. A new high performance liquid chromatography (HPLC) method is described that permits the accurate and specific measurement of glycerol SA. The method involves isolation of glycerol from plasma and the formation of a tribenzoyl derivative. Glycerol rate of appearance was measured in five human volunteers using both [2-3H]glycerol and [2H5] glycerol. There was close agreement between the glycerol appearance rates measured using the two approaches (1.66 ± 0.14 vs. 1.70 ± 0.10 μmol · kg-1 min-1, respectively, P = NS). This HPLC method offers improved specificity over existing methods of measuring glycerol turnover using radiotracers.

Original languageEnglish (US)
Pages (from-to)1106-1110
Number of pages5
JournalJournal of Lipid Research
Volume39
Issue number5
StatePublished - May 1 1998

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Keywords

  • HPLC
  • Lipolysis
  • Radioisotopes
  • Stable isotopes

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

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