Measurement of plasma acetate kinetics using high-performance liquid chromatography

M. Persson, B. Bleiberg, D. Kiss, J. Miles

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Previous studies suggest that plasma acetate may be an important fuel in man, accounting for ∼ 10% of energy expenditure. Available methods for the determination of plasma acetate kinetics are difficult and time consuming. We describe here a procedure for the determination of plasma acetate concentration and specific activity using automated high-performance liquid chromatography that is precise and sensitive and accommodates large numbers of samples. The procedure involves extraction from plasma with diethyl ether, derivatization with bromoacetophenone, and separation on a C-18 reversed-phase column. The specific activities of d-β-hydroxybutyrate and lactate can also be determined. Acetate turnover was measured in four dogs and was similar to that previously reported in sheep and humans. Transport of [14C]acetate into red blood cells was negligible.

Original languageEnglish (US)
Pages (from-to)149-153
Number of pages5
JournalAnalytical Biochemistry
Volume198
Issue number1
DOIs
StatePublished - Oct 1991

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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