Measurement of O2 consumption in isolated organs without venous cannulation

T. R. DeGrado, J. E. Holden, C. K. Ng, D. M. Raffel, S. J. Gatley

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


A new two-sensor technique for measurement of O2 consumption in isolated organs without venous cannulation was successfully applied to the isolated rat heart. Because this technique eliminates the net exchange of O2 between venous effluent and the environment, measurement of the O2 concentration is conveniently made by use of a polarographic sensor in a collected pool of effluent at the bottom of a closed organ chamber. The method was validated against conventional techniques using cannulation of the pulmonary artery. The two-sensor technique allows O2 consumption measurements to be made in isolated organ preparations in which representative venous cannulation is prohibitively difficult, for example in organs with multiple venous drains, or those in which cannulation would be expected to cause excessive perturbation of physiological status.

Original languageEnglish (US)
Pages (from-to)1316-1320
Number of pages5
JournalJournal of applied physiology
Issue number3
StatePublished - 1989

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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