Measurement of human skeletal muscle oxidative capacity by 31P-MR spectroscopy: A cross-validation with in vitro measurements

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Purpose: To cross-validate skeletal muscle oxidative capacity measured by 31P-MR spectroscopy with in vitro measurements of oxidative capacity in mitochondria isolated from muscle biopsies of the same muscle group in 18 healthy adults. Materials and Methods: Oxidative capacity in vivo was determined from PCr recovery kinetics following a 30-s maximal isometric knee extension. State 3 respiration was measured in isolated mitochondria using high-resolution respirometry. A second cohort of 10 individuals underwent two 31P-MRS testing sessions to assess the test-retest reproducibility of the method. Results: Overall, the in vivo and in vitro methods were well-correlated (r = 0.66-0.72) and showed good agreement by Bland Altman plots. Excellent reproducibility was observed for the PCr recovery rate constant (CV = 4.6%; ICC = 0.85) and calculated oxidative capacity (CV = 3.4%; ICC = 0.83). Conclusion: These results indicate that 31P-MRS corresponds well with gold-standard in vitro measurements and is highly reproducible.

Original languageEnglish (US)
Pages (from-to)1143-1150
Number of pages8
JournalJournal of Magnetic Resonance Imaging
Volume34
Issue number5
DOIs
StatePublished - Nov 2011

Fingerprint

Skeletal Muscle
Magnetic Resonance Spectroscopy
Muscle Mitochondrion
Gold
Knee
Mitochondria
Respiration
Biopsy
Muscles
In Vitro Techniques

Keywords

  • high-resolution respirometry
  • mitochondria
  • oxidative capacity
  • phosphocreatine recovery
  • skeletal muscle

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

@article{50a485e79fba4318b97e2f8bd44ea7c2,
title = "Measurement of human skeletal muscle oxidative capacity by 31P-MR spectroscopy: A cross-validation with in vitro measurements",
abstract = "Purpose: To cross-validate skeletal muscle oxidative capacity measured by 31P-MR spectroscopy with in vitro measurements of oxidative capacity in mitochondria isolated from muscle biopsies of the same muscle group in 18 healthy adults. Materials and Methods: Oxidative capacity in vivo was determined from PCr recovery kinetics following a 30-s maximal isometric knee extension. State 3 respiration was measured in isolated mitochondria using high-resolution respirometry. A second cohort of 10 individuals underwent two 31P-MRS testing sessions to assess the test-retest reproducibility of the method. Results: Overall, the in vivo and in vitro methods were well-correlated (r = 0.66-0.72) and showed good agreement by Bland Altman plots. Excellent reproducibility was observed for the PCr recovery rate constant (CV = 4.6{\%}; ICC = 0.85) and calculated oxidative capacity (CV = 3.4{\%}; ICC = 0.83). Conclusion: These results indicate that 31P-MRS corresponds well with gold-standard in vitro measurements and is highly reproducible.",
keywords = "high-resolution respirometry, mitochondria, oxidative capacity, phosphocreatine recovery, skeletal muscle",
author = "Lanza, {Ian R} and Sumit Bhagra and Nair, {K Sreekumaran} and Port, {John D}",
year = "2011",
month = "11",
doi = "10.1002/jmri.22733",
language = "English (US)",
volume = "34",
pages = "1143--1150",
journal = "Journal of Magnetic Resonance Imaging",
issn = "1053-1807",
publisher = "John Wiley and Sons Inc.",
number = "5",

}

TY - JOUR

T1 - Measurement of human skeletal muscle oxidative capacity by 31P-MR spectroscopy

T2 - A cross-validation with in vitro measurements

AU - Lanza, Ian R

AU - Bhagra, Sumit

AU - Nair, K Sreekumaran

AU - Port, John D

PY - 2011/11

Y1 - 2011/11

N2 - Purpose: To cross-validate skeletal muscle oxidative capacity measured by 31P-MR spectroscopy with in vitro measurements of oxidative capacity in mitochondria isolated from muscle biopsies of the same muscle group in 18 healthy adults. Materials and Methods: Oxidative capacity in vivo was determined from PCr recovery kinetics following a 30-s maximal isometric knee extension. State 3 respiration was measured in isolated mitochondria using high-resolution respirometry. A second cohort of 10 individuals underwent two 31P-MRS testing sessions to assess the test-retest reproducibility of the method. Results: Overall, the in vivo and in vitro methods were well-correlated (r = 0.66-0.72) and showed good agreement by Bland Altman plots. Excellent reproducibility was observed for the PCr recovery rate constant (CV = 4.6%; ICC = 0.85) and calculated oxidative capacity (CV = 3.4%; ICC = 0.83). Conclusion: These results indicate that 31P-MRS corresponds well with gold-standard in vitro measurements and is highly reproducible.

AB - Purpose: To cross-validate skeletal muscle oxidative capacity measured by 31P-MR spectroscopy with in vitro measurements of oxidative capacity in mitochondria isolated from muscle biopsies of the same muscle group in 18 healthy adults. Materials and Methods: Oxidative capacity in vivo was determined from PCr recovery kinetics following a 30-s maximal isometric knee extension. State 3 respiration was measured in isolated mitochondria using high-resolution respirometry. A second cohort of 10 individuals underwent two 31P-MRS testing sessions to assess the test-retest reproducibility of the method. Results: Overall, the in vivo and in vitro methods were well-correlated (r = 0.66-0.72) and showed good agreement by Bland Altman plots. Excellent reproducibility was observed for the PCr recovery rate constant (CV = 4.6%; ICC = 0.85) and calculated oxidative capacity (CV = 3.4%; ICC = 0.83). Conclusion: These results indicate that 31P-MRS corresponds well with gold-standard in vitro measurements and is highly reproducible.

KW - high-resolution respirometry

KW - mitochondria

KW - oxidative capacity

KW - phosphocreatine recovery

KW - skeletal muscle

UR - http://www.scopus.com/inward/record.url?scp=80054720141&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80054720141&partnerID=8YFLogxK

U2 - 10.1002/jmri.22733

DO - 10.1002/jmri.22733

M3 - Article

C2 - 22006551

AN - SCOPUS:80054720141

VL - 34

SP - 1143

EP - 1150

JO - Journal of Magnetic Resonance Imaging

JF - Journal of Magnetic Resonance Imaging

SN - 1053-1807

IS - 5

ER -