Measurement of gut permeability using fluorescent tracer agent technology

Richard B. Dorshow, Carla Hall-Moore, Nurmohammad Shaikh, Michael R. Talcott, William Alvis Faubion, Thomas E. Rogers, Jeng Jong Shieh, Martin P. Debreczeny, James R. Johnson, Roy B. Dyer, Ravinder Jit Singh, Phillip I. Tarr

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The healthy gut restricts macromolecular and bacterial movement across tight junctions, while increased intestinal permeability accompanies many intestinal disorders. Dual sugar absorption tests, which measure intestinal permeability in humans, present challenges. Therefore, we asked if enterally administered fluorescent tracers could ascertain mucosal integrity, because transcutaneous measurement of differentially absorbed molecules could enable specimen-free evaluation of permeability. We induced small bowel injury in rats using high-(15 mg/kg), intermediate-(10 mg/kg), and low-(5 mg/kg) dose indomethacin. Then, we compared urinary ratios of enterally administered fluorescent tracers MB-402 and MB-301 to urinary ratios of sugar tracers lactulose and rhamnose. We also tested the ability of transcutaneous sensors to measure the ratios of absorbed fluorophores. Urinary fluorophore and sugar ratios reflect gut injury in an indomethacin dose dependent manner. The fluorophores generated smooth curvilinear ratio trajectories with wide dynamic ranges. The more chaotic sugar ratios had narrower dynamic ranges. Fluorophore ratios measured through the skin distinguished indomethacin-challenged from same day control rats. Enterally administered fluorophores can identify intestinal injury in a rat model. Fluorophore ratios are measureable through the skin, obviating drawbacks of dual sugar absorption tests. Pending validation, this technology should be considered for human use.

Original languageEnglish (US)
Article number10888
JournalScientific Reports
Volume7
Issue number1
DOIs
StatePublished - Dec 1 2017

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Fluorescent Dyes
Indomethacin
Permeability
Technology
Wounds and Injuries
Lactulose
Rhamnose
Skin
Tight Junctions

ASJC Scopus subject areas

  • General

Cite this

Dorshow, R. B., Hall-Moore, C., Shaikh, N., Talcott, M. R., Faubion, W. A., Rogers, T. E., ... Tarr, P. I. (2017). Measurement of gut permeability using fluorescent tracer agent technology. Scientific Reports, 7(1), [10888]. https://doi.org/10.1038/s41598-017-09971-y

Measurement of gut permeability using fluorescent tracer agent technology. / Dorshow, Richard B.; Hall-Moore, Carla; Shaikh, Nurmohammad; Talcott, Michael R.; Faubion, William Alvis; Rogers, Thomas E.; Shieh, Jeng Jong; Debreczeny, Martin P.; Johnson, James R.; Dyer, Roy B.; Singh, Ravinder Jit; Tarr, Phillip I.

In: Scientific Reports, Vol. 7, No. 1, 10888, 01.12.2017.

Research output: Contribution to journalArticle

Dorshow, RB, Hall-Moore, C, Shaikh, N, Talcott, MR, Faubion, WA, Rogers, TE, Shieh, JJ, Debreczeny, MP, Johnson, JR, Dyer, RB, Singh, RJ & Tarr, PI 2017, 'Measurement of gut permeability using fluorescent tracer agent technology', Scientific Reports, vol. 7, no. 1, 10888. https://doi.org/10.1038/s41598-017-09971-y
Dorshow, Richard B. ; Hall-Moore, Carla ; Shaikh, Nurmohammad ; Talcott, Michael R. ; Faubion, William Alvis ; Rogers, Thomas E. ; Shieh, Jeng Jong ; Debreczeny, Martin P. ; Johnson, James R. ; Dyer, Roy B. ; Singh, Ravinder Jit ; Tarr, Phillip I. / Measurement of gut permeability using fluorescent tracer agent technology. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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