Measurement of bone blood flow using the hydrogen washout technique - Part I: Quantitative evaluation of tissue perfusion in the laboratory rat

Michael Pelzer, Mikko Larsen, Patricia F. Friedrich, Allen T. Bishop

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

The measurement of blood flow in bone is of interest in both clinical and experimental settings. Such determinations would ideally provide accurate, quantitative, and reproducible data with relatively simple and safe technology, even in the small bones of experimental animals. Methods that provide absolute or quantitative measurements include positron emission tomography, "isotope fractioning" using radioactive or fluorescent-labeled microspheres, and measurement of the rate of washout of diffusible tracers delivered either by injection or inhalation. In this article, we describe in detail a modification of the original Whiteside hydrogen washout technique, using modern hydrogen sensors, a micromanipulator for probe placement, and custom software that greatly simplifies blood flow determination and is effective in the small bones of experimental animals.

Original languageEnglish (US)
Pages (from-to)741-745
Number of pages5
JournalJournal of Orthopaedic Research
Volume26
Issue number6
DOIs
StatePublished - Jun 2008

Keywords

  • Blood flow
  • Bone
  • Hydrogen washout

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

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