Measles virus spread and pathogenesis in genetically modified mice

Branka Mrkic, Jovan Pavlovic, Thomas Rülicke, Pietro Volpe, Christian J. Buchholz, Dennis Hourcade, John P. Atkinson, Adriano Aguzzi, Roberto Cattaneo

Research output: Contribution to journalArticle

226 Citations (Scopus)

Abstract

Attenuated Edmonston measles virus (MV-Edm) is not pathogenic in standard mice. We show here that MV-Edm inoculated via the natural respiratory route has a limited propagation in the lungs of mice with a targeted mutation inactivating the alpha/beta interferon receptor. A high dose of MV-Edm administered intracerebrally is lethal for about half of these mice. To study the consequences of the availability of a high-affinity receptor for MV propagation, we generated alpha/beta interferon-defective mice expressing human CD46 with human-like tissue specificity. Intranasal infection of these mice with MV-Edm resulted in enhanced spread to the lungs and more prominent inflammatory response. Virus replication was also detected in peripheral blood mononuclear cells, the spleen, and the liver. Moreover, intracerebral inoculation of adult animals with low MV-Edm doses caused encephalitis with almost inevitably lethal outcome. We conclude that in mice alpha/beta interferon controls MV infection and that a high-affinity receptor facilitates, but is not strictly required for, MV spread and pathogenesis.

Original languageEnglish (US)
Pages (from-to)7420-7427
Number of pages8
JournalJournal of Virology
Volume72
Issue number9
StatePublished - Sep 1998
Externally publishedYes

Fingerprint

Measles virus
pathogenesis
interferon-beta
mice
Interferon-beta
lethal genes
Interferon-alpha
receptors
lungs
Interferon alpha-beta Receptor
Lung
Organ Specificity
Encephalitis
encephalitis
Infection Control
Virus Replication
mononuclear leukocytes
virus replication
dosage
infection

ASJC Scopus subject areas

  • Immunology

Cite this

Mrkic, B., Pavlovic, J., Rülicke, T., Volpe, P., Buchholz, C. J., Hourcade, D., ... Cattaneo, R. (1998). Measles virus spread and pathogenesis in genetically modified mice. Journal of Virology, 72(9), 7420-7427.

Measles virus spread and pathogenesis in genetically modified mice. / Mrkic, Branka; Pavlovic, Jovan; Rülicke, Thomas; Volpe, Pietro; Buchholz, Christian J.; Hourcade, Dennis; Atkinson, John P.; Aguzzi, Adriano; Cattaneo, Roberto.

In: Journal of Virology, Vol. 72, No. 9, 09.1998, p. 7420-7427.

Research output: Contribution to journalArticle

Mrkic, B, Pavlovic, J, Rülicke, T, Volpe, P, Buchholz, CJ, Hourcade, D, Atkinson, JP, Aguzzi, A & Cattaneo, R 1998, 'Measles virus spread and pathogenesis in genetically modified mice', Journal of Virology, vol. 72, no. 9, pp. 7420-7427.
Mrkic B, Pavlovic J, Rülicke T, Volpe P, Buchholz CJ, Hourcade D et al. Measles virus spread and pathogenesis in genetically modified mice. Journal of Virology. 1998 Sep;72(9):7420-7427.
Mrkic, Branka ; Pavlovic, Jovan ; Rülicke, Thomas ; Volpe, Pietro ; Buchholz, Christian J. ; Hourcade, Dennis ; Atkinson, John P. ; Aguzzi, Adriano ; Cattaneo, Roberto. / Measles virus spread and pathogenesis in genetically modified mice. In: Journal of Virology. 1998 ; Vol. 72, No. 9. pp. 7420-7427.
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