Measles vector as a multigene delivery platform facilitating iPSC reprogramming

Qi Wang, Alanna Vossen, Yasuhiro Ikeda, Patricia Devaux

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Induced pluripotent stem cells (iPSCs) provide a unique platform for individualized cell therapy approaches. Currently, episomal DNA, mRNA, and Sendai virus-based RNA reprogramming systems are widely used to generate iPSCs. However, they all rely on the use of multiple (three to six) components (vectors/plasmids/mRNAs) leading to the production of partially reprogrammed cells, reducing the efficiency of the systems. We produced a one-cycle measles virus (MV) vector by substituting the viral attachment protein gene with the green fluorescent protein (GFP) gene. Here, we present a highly efficient multi-transgene delivery system based on a vaccine strain of MV, a non-integrating RNA virus that has a long-standing safety record in humans. Introduction of the four reprogramming factors OCT4, SOX2, KLF4, and cMYC via a single, “one-cycle” MV vector efficiently reprogrammed human somatic cells into iPSCs, whereas MV vector genomes are rapidly eliminated in derived iPSCs. Our MV vector system offers a new reprogramming platform for genomic modification-free iPSCs amenable for clinical translation.

Original languageEnglish (US)
Pages (from-to)151-164
Number of pages14
JournalGene Therapy
Volume26
Issue number5
DOIs
StatePublished - May 1 2019

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics

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