Maximum likelihood method for the analysis of time-resolved fluorescence decay curves

Željko Bajzer, Terry M. Therneau, Joseph C. Sharp, Franklin G. Prendergast

Research output: Contribution to journalArticle

42 Scopus citations

Abstract

The usefulness of fluorescence techniques for the study of macromolecular structure and dynamics depends on the accuracy and sensitivity of the methods used for data analysis. Many methods for data analysis have been proposed and used, but little attention has been paid to the maximum likelihood method, generally known as the most powerful statistical method for parameter estimation. In this paper we study the properties and behavior of maximum likelihood estimates by using simulated fluorescence intensity decay data. We show that the maximum likelihood method provides generally more accurate estimates of lifetimes and fractions than does the standard least-squares approach especially when the lifetime ratios between individual components are small. Three novelties to the field of fluorescence decay analysis are also introduced and studied in this paper: a) discretization of the convolution integral based on the generalized integral mean value theorem: b) the likelihood ratio test as a tool to determine the number of exponential decay components in a given decay profile; and c) separability and detectability indices which provide measures on how accurately, a particular decay component can be detected. Based on the experience gained from this and from our previous study of the Padé-Laplace method, we make some recommendations on how the complex problem of deconvolution and parameter estimation of multiexponential functions might be approached in an experimental setting.

Original languageEnglish (US)
Pages (from-to)247-262
Number of pages16
JournalEuropean Biophysics Journal
Volume20
Issue number5
DOIs
StatePublished - Dec 1 1991

Keywords

  • Fluorescence decay
  • Maximum likelihood

ASJC Scopus subject areas

  • Biophysics

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