Maternal T cells in the human placental villi support an allograft response during noninfectious villitis

Elizabeth Ann L. Enninga, Patrick Raber, Reade A. Quinton, Rodrigo Ruano, Nadia Ikumi, Clive M. Gray, Erica L. Johnson, Rana Chakraborty, Sarah E. Kerr

Research output: Contribution to journalArticle

Abstract

During human pregnancy, proinflammatory responses in the placenta can cause severe fetal complications, including growth restriction, preterm birth, and stillbirth. Villitis of unknown etiology (VUE), an inflammatory condition characterized by the infiltration of maternal CD8+ T cells into the placenta, is hypothesized to be secondary to either a tissue rejection response to the haploidentical fetus or from an undiagnosed infection. In this study, we characterized the global TCR b-chain profile in human T cells isolated from placentae diagnosed with VUE compared with control and infectious villitis–placentae by immunoSEQ. Immunosequencing demonstrated that VUE is driven predominantly by maternal T cell infiltration, which is significantly different from controls and infectious cases; however, these T cell clones show very little overlap between subjects. Mapping TCR clones to common viral epitopes (CMV, EBV, and influenza A) demonstrated that Ag specificity in VUE was equal to controls and significantly lower than CMV-specific clones in infectious villitis. Our data indicate VUE represents an allograft response, not an undetected infection. These observations support the development of screening methods to predict those at risk for VUE and the use of specific immunomodulatory therapies during gestation to improve outcomes in affected fetuses.

Original languageEnglish (US)
Pages (from-to)2931-2939
Number of pages9
JournalJournal of Immunology
Volume204
Issue number11
DOIs
StatePublished - Jun 1 2020

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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  • Cite this

    Enninga, E. A. L., Raber, P., Quinton, R. A., Ruano, R., Ikumi, N., Gray, C. M., Johnson, E. L., Chakraborty, R., & Kerr, S. E. (2020). Maternal T cells in the human placental villi support an allograft response during noninfectious villitis. Journal of Immunology, 204(11), 2931-2939. https://doi.org/10.4049/jimmunol.1901297