Marrow stromal osteoblast function on a poly(propylene fumarate)/β-tricalcium phosphate biodegradable orthopaedic composite

Susan J. Peter, Lichun Lu, Daniel J. Kim, Antonios G. Mikos

Research output: Contribution to journalArticle

136 Citations (Scopus)

Abstract

The objective of this study was to assess the osteoconductivity of a poly(propylene fumarate)/β-tricalcium phosphate (PPF/β-TCP) composite in vitro. We examined whether primary rat marrow stromal cells would attach, proliferate, and express differentiated osteoblastic function when seeded on PPF/β-TCP substrates. Attachment studies showed that a confluent monolayer of cells had adhered to the substrates within an 8h time frame for marrow stromal cells seeded at confluent numbers. Proliferation and differentiated function of the cells were then investigated for a period of 4 weeks for an initial seeding density of 42 000cells/cm2. Rapid proliferation during the first 24h as determined by 3H-thymidine incorporation was mirrored by an initial rapid increase in total cell number by DNA assay. A lower proliferation rate and a gradual increase in cell number persisted for the remainder of the study, resulting in a final cell number of 128 000cells/cm2. Differentiated cell function was assessed by measuring alkaline phosphatase (ALP) activity and osteocalcin (OC) production throughout the time course. Both markers of osteoblastic differentiation increased significantly over a 4-week period. By day 28, cells grown on PPF/β-TCP reached a maximal ALP activity of 11 (±1)x10-7μmol/min/cell, while the OC production reached 40 (±1)x10-6ng/cell. These data show that a PPF/β-TCP composite exhibits in vitro osteoconductivity similar to or better than that of control tissue culture polystyrene. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)1207-1213
Number of pages7
JournalBiomaterials
Volume21
Issue number12
DOIs
StatePublished - Jun 2000
Externally publishedYes

Fingerprint

Osteoblasts
Orthopedics
Fumarates
Polypropylenes
Phosphates
Bone Marrow
Propylene
Osteocalcin
Phosphatases
Composite materials
Alkaline Phosphatase
Cell Count
Cells
Stromal Cells
Tissue culture
Polystyrenes
Substrates
Thymidine
Rats
Monolayers

Keywords

  • β-Tricalcium phosphate (β-TCP)
  • Marrow stromal cells
  • Orthopaedic scaffold
  • Osteoblastic differentiation
  • Osteoconductivity
  • Poly(propylene fumarate) (PPF)

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Marrow stromal osteoblast function on a poly(propylene fumarate)/β-tricalcium phosphate biodegradable orthopaedic composite. / Peter, Susan J.; Lu, Lichun; Kim, Daniel J.; Mikos, Antonios G.

In: Biomaterials, Vol. 21, No. 12, 06.2000, p. 1207-1213.

Research output: Contribution to journalArticle

Peter, Susan J. ; Lu, Lichun ; Kim, Daniel J. ; Mikos, Antonios G. / Marrow stromal osteoblast function on a poly(propylene fumarate)/β-tricalcium phosphate biodegradable orthopaedic composite. In: Biomaterials. 2000 ; Vol. 21, No. 12. pp. 1207-1213.
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