In order to study angiogenesis and vascular networks in histology samples, an appropriate stain for endothelial cell surfaces is required. This chapter reviews the various possible antibodies used to identify blood and lymph vessel endothelium, and other vascular markers in both immune histochemical and immunofluorescent studies. Two categories of human endothelial cell-specific antibodies are commonly used: the pan-endothelial cell markers and antibodies that bind selectively to activated or proliferating endothelium. The chapter concludes with a review and discussion of potential clinical uses of angiogenesis quantification.
- Digital pathology
- Microvessel density
- Tissue image analysis on intact
ASJC Scopus subject areas
- Molecular Medicine
- Pharmacology, Toxicology and Pharmaceutics(all)
- Pharmacology (medical)