Mapping of the chromosome 19 q-arm glioma tumor suppressor gene using fluorescence in situ hybridization and novel microsatellite markers

Justin S. Smith, Issei Tachibana, Hyun K. Lee, Junqi Qian, Ute Pohl, Harvey W. Mohrenweiser, Thomas J. Borell, Sandra M. Hosek, Cheryl L. Soderberg, Andreas Von Deimling, Arie Perry, Bernd W. Scheithauer, David N. Louis, Robert Brian Jenkins

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Abstract

Allelic loss of chromosome arm 19q is a frequent event in human diffuse glioma, suggesting the presence of a tumor suppressor gene. Previous loss of heterozygosity (LOH) analyses have mapped this gene to a 1.4-megabase interval, between the genetic markers D19S412 and STD. Further narrowing of this interval has been limited by the resolution of mapped polymorphic markers. In the present study, we have used genomic clones mapped to 19q as fluorescence in situ hybridization (FISH) probes to map the breakpoints of 13 gliomas with 19q13.3 deletion boundaries. In addition, we have developed three new polymorphic microsatellite markers (D19S1180, D19S1181, and D19S1182) that map between D19S412 and STD and have used these new markers to identify two gliomas with small deletions between the D19S412 and STD markers. Collectively, these data suggest that the region of common deletion may be as narrow as 150 kb and should facilitate future efforts to identify the glioma 19q tumor suppressor gene. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish (US)
Pages (from-to)16-25
Number of pages10
JournalGenes Chromosomes and Cancer
Volume29
Issue number1
DOIs
StatePublished - Sep 2000

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ASJC Scopus subject areas

  • Cancer Research
  • Genetics

Cite this

Smith, J. S., Tachibana, I., Lee, H. K., Qian, J., Pohl, U., Mohrenweiser, H. W., Borell, T. J., Hosek, S. M., Soderberg, C. L., Von Deimling, A., Perry, A., Scheithauer, B. W., Louis, D. N., & Jenkins, R. B. (2000). Mapping of the chromosome 19 q-arm glioma tumor suppressor gene using fluorescence in situ hybridization and novel microsatellite markers. Genes Chromosomes and Cancer, 29(1), 16-25. https://doi.org/10.1002/1098-2264(2000)9999:9999<::AID-GCC1007>3.0.CO;2-I