Mapping lubricin in canine musculoskeletal tissues

Yulong Sun, Evelyn J. Berger, Chunfeng D Zhao, Kai N. An, Peter C Amadio, Gregory Jay

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Lubricin, also known as superficial zone protein or PRG4, has many distinct biological functions, including lubrication, antiadhesion, and as a regulator of cell growth. This study investigated lubricin in canine musculoskeletal tissues using RT-PCR and immunohistochemistry. One or more variants were noted in canine flexor digitorum profundus (FDP) tendon, Achilles tendon, patellar tendon, A2 pulley, anterior cruciate ligament (ACL), knee lateral collateral ligament (LCL), articular cartilage, meniscus, muscle, and skin. We found 6 N-terminal lubricin splicing variants. The variants with larger sizes were identified in FDP tendon, ACL, LCL, A2 pulley, and cartilage. Lubricin was distributed both on the tissue surfaces and at the interface of fiber bundles within tissues, but this distribution varied by tissue type. We conclude that lubricin is present in many tissues; variations in splicing and physical distribution suggest that the variants of lubricin may play different roles in different locations.

Original languageEnglish (US)
Pages (from-to)215-221
Number of pages7
JournalConnective Tissue Research
Volume47
Issue number4
DOIs
StatePublished - Jul 2006

Fingerprint

Canidae
Ligaments
Tendons
Tissue
Ankle Lateral Ligament
Pulleys
Anterior Cruciate Ligament
Cartilage
varespladib methyl
Lubrication
Patellar Ligament
Achilles Tendon
Cell growth
Articular Cartilage
Tissue Distribution
Muscle
lubricin
Knee
Skin
Immunohistochemistry

Keywords

  • Lubricin
  • Musculoskeletal tissues
  • Tanine

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Immunology
  • Nephrology
  • Orthopedics and Sports Medicine

Cite this

Mapping lubricin in canine musculoskeletal tissues. / Sun, Yulong; Berger, Evelyn J.; Zhao, Chunfeng D; An, Kai N.; Amadio, Peter C; Jay, Gregory.

In: Connective Tissue Research, Vol. 47, No. 4, 07.2006, p. 215-221.

Research output: Contribution to journalArticle

Sun, Yulong ; Berger, Evelyn J. ; Zhao, Chunfeng D ; An, Kai N. ; Amadio, Peter C ; Jay, Gregory. / Mapping lubricin in canine musculoskeletal tissues. In: Connective Tissue Research. 2006 ; Vol. 47, No. 4. pp. 215-221.
@article{1ee9507fdafa443687300744e1e811b1,
title = "Mapping lubricin in canine musculoskeletal tissues",
abstract = "Lubricin, also known as superficial zone protein or PRG4, has many distinct biological functions, including lubrication, antiadhesion, and as a regulator of cell growth. This study investigated lubricin in canine musculoskeletal tissues using RT-PCR and immunohistochemistry. One or more variants were noted in canine flexor digitorum profundus (FDP) tendon, Achilles tendon, patellar tendon, A2 pulley, anterior cruciate ligament (ACL), knee lateral collateral ligament (LCL), articular cartilage, meniscus, muscle, and skin. We found 6 N-terminal lubricin splicing variants. The variants with larger sizes were identified in FDP tendon, ACL, LCL, A2 pulley, and cartilage. Lubricin was distributed both on the tissue surfaces and at the interface of fiber bundles within tissues, but this distribution varied by tissue type. We conclude that lubricin is present in many tissues; variations in splicing and physical distribution suggest that the variants of lubricin may play different roles in different locations.",
keywords = "Lubricin, Musculoskeletal tissues, Tanine",
author = "Yulong Sun and Berger, {Evelyn J.} and Zhao, {Chunfeng D} and An, {Kai N.} and Amadio, {Peter C} and Gregory Jay",
year = "2006",
month = "7",
doi = "10.1080/03008200600846754",
language = "English (US)",
volume = "47",
pages = "215--221",
journal = "Connective Tissue Research",
issn = "0300-8207",
publisher = "Informa Healthcare",
number = "4",

}

TY - JOUR

T1 - Mapping lubricin in canine musculoskeletal tissues

AU - Sun, Yulong

AU - Berger, Evelyn J.

AU - Zhao, Chunfeng D

AU - An, Kai N.

AU - Amadio, Peter C

AU - Jay, Gregory

PY - 2006/7

Y1 - 2006/7

N2 - Lubricin, also known as superficial zone protein or PRG4, has many distinct biological functions, including lubrication, antiadhesion, and as a regulator of cell growth. This study investigated lubricin in canine musculoskeletal tissues using RT-PCR and immunohistochemistry. One or more variants were noted in canine flexor digitorum profundus (FDP) tendon, Achilles tendon, patellar tendon, A2 pulley, anterior cruciate ligament (ACL), knee lateral collateral ligament (LCL), articular cartilage, meniscus, muscle, and skin. We found 6 N-terminal lubricin splicing variants. The variants with larger sizes were identified in FDP tendon, ACL, LCL, A2 pulley, and cartilage. Lubricin was distributed both on the tissue surfaces and at the interface of fiber bundles within tissues, but this distribution varied by tissue type. We conclude that lubricin is present in many tissues; variations in splicing and physical distribution suggest that the variants of lubricin may play different roles in different locations.

AB - Lubricin, also known as superficial zone protein or PRG4, has many distinct biological functions, including lubrication, antiadhesion, and as a regulator of cell growth. This study investigated lubricin in canine musculoskeletal tissues using RT-PCR and immunohistochemistry. One or more variants were noted in canine flexor digitorum profundus (FDP) tendon, Achilles tendon, patellar tendon, A2 pulley, anterior cruciate ligament (ACL), knee lateral collateral ligament (LCL), articular cartilage, meniscus, muscle, and skin. We found 6 N-terminal lubricin splicing variants. The variants with larger sizes were identified in FDP tendon, ACL, LCL, A2 pulley, and cartilage. Lubricin was distributed both on the tissue surfaces and at the interface of fiber bundles within tissues, but this distribution varied by tissue type. We conclude that lubricin is present in many tissues; variations in splicing and physical distribution suggest that the variants of lubricin may play different roles in different locations.

KW - Lubricin

KW - Musculoskeletal tissues

KW - Tanine

UR - http://www.scopus.com/inward/record.url?scp=33749142312&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33749142312&partnerID=8YFLogxK

U2 - 10.1080/03008200600846754

DO - 10.1080/03008200600846754

M3 - Article

C2 - 16987753

AN - SCOPUS:33749142312

VL - 47

SP - 215

EP - 221

JO - Connective Tissue Research

JF - Connective Tissue Research

SN - 0300-8207

IS - 4

ER -