TY - JOUR
T1 - Mapping lubricin in canine musculoskeletal tissues
AU - Sun, Yulong
AU - Berger, Evelyn J.
AU - Zhao, Chunfeng
AU - An, Kai Nan
AU - Amadio, Peter C.
AU - Jay, Gregory
N1 - Funding Information:
We thank Dr. Thomas Schmid (Department of Biochemistry, Rush Medical College at Rush-Presbyterian-St. Luke’s Medical Center, Chicago, IL, USA) for kindly providing lubricin monoclonal antibody MAb S6.79, and David Hebrink, Dr. Jim Maher, and Dr. Toshikazu Tanaka for their excellent technical assistance. This study was supported by NIH grant AR44391.
PY - 2006/7
Y1 - 2006/7
N2 - Lubricin, also known as superficial zone protein or PRG4, has many distinct biological functions, including lubrication, antiadhesion, and as a regulator of cell growth. This study investigated lubricin in canine musculoskeletal tissues using RT-PCR and immunohistochemistry. One or more variants were noted in canine flexor digitorum profundus (FDP) tendon, Achilles tendon, patellar tendon, A2 pulley, anterior cruciate ligament (ACL), knee lateral collateral ligament (LCL), articular cartilage, meniscus, muscle, and skin. We found 6 N-terminal lubricin splicing variants. The variants with larger sizes were identified in FDP tendon, ACL, LCL, A2 pulley, and cartilage. Lubricin was distributed both on the tissue surfaces and at the interface of fiber bundles within tissues, but this distribution varied by tissue type. We conclude that lubricin is present in many tissues; variations in splicing and physical distribution suggest that the variants of lubricin may play different roles in different locations.
AB - Lubricin, also known as superficial zone protein or PRG4, has many distinct biological functions, including lubrication, antiadhesion, and as a regulator of cell growth. This study investigated lubricin in canine musculoskeletal tissues using RT-PCR and immunohistochemistry. One or more variants were noted in canine flexor digitorum profundus (FDP) tendon, Achilles tendon, patellar tendon, A2 pulley, anterior cruciate ligament (ACL), knee lateral collateral ligament (LCL), articular cartilage, meniscus, muscle, and skin. We found 6 N-terminal lubricin splicing variants. The variants with larger sizes were identified in FDP tendon, ACL, LCL, A2 pulley, and cartilage. Lubricin was distributed both on the tissue surfaces and at the interface of fiber bundles within tissues, but this distribution varied by tissue type. We conclude that lubricin is present in many tissues; variations in splicing and physical distribution suggest that the variants of lubricin may play different roles in different locations.
KW - Lubricin
KW - Musculoskeletal tissues
KW - Tanine
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U2 - 10.1080/03008200600846754
DO - 10.1080/03008200600846754
M3 - Article
C2 - 16987753
AN - SCOPUS:33749142312
SN - 0300-8207
VL - 47
SP - 215
EP - 221
JO - Connective Tissue Research
JF - Connective Tissue Research
IS - 4
ER -