Mapping class I gene sequences in the major histocompatibility complex

Larry R Pease, S. G. Nathenson, L. A. Leinwand

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The class I glycoproteins of the major histocompatibility complex (MHC) are products of closely linked genes on the 17th chromosome of the mouse. Four highly homologous cell surface proteins, K, D, L, and Qa-2 (refs 1-4), belonging to this multigene family have been analysed. Several additional members, M, R, TL, are indicated by biochemical data and recent serological data suggest the family is larger. Recent reports using recombinant DNA technology suggest that the class I system is an extensive multigene family. In this report we describe results of experiments using restriction enzyme digestion of DNA from a group of standard, congeneic and MHC recombinant mouse strains followed by hybridization to H-2 cDNA probes. Particular bands of hybridization can be assigned to specific H-2 haplotypes and, therefore, to the MHC region of the 17th chromosome. A number of the MHC polymorphic bands have been assigned to genetic map positions in the K and D-L regions of the H-2 complex. Additional class I gene sequences map outside the boundaries of the traditional H-2 complex (K, I, S, D), into the Qa-T1a region. At least one sequence is centromeric to the K locus. Further, amino terminal and carboxy terminal probes show different restriction patterns, suggesting that the exons corresponding to the different domains of the class I glycoproteins may have different evolutionary histories.

Original languageEnglish (US)
Pages (from-to)382-385
Number of pages4
JournalNature
Volume298
Issue number5872
StatePublished - 1982
Externally publishedYes

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MHC Class I Genes
Major Histocompatibility Complex
Multigene Family
Glycoproteins
Chromosomes
Recombinant DNA
DNA Restriction Enzymes
Haplotypes
Digestion
Exons
Membrane Proteins
Complementary DNA
Technology
Genes

ASJC Scopus subject areas

  • General

Cite this

Pease, L. R., Nathenson, S. G., & Leinwand, L. A. (1982). Mapping class I gene sequences in the major histocompatibility complex. Nature, 298(5872), 382-385.

Mapping class I gene sequences in the major histocompatibility complex. / Pease, Larry R; Nathenson, S. G.; Leinwand, L. A.

In: Nature, Vol. 298, No. 5872, 1982, p. 382-385.

Research output: Contribution to journalArticle

Pease, LR, Nathenson, SG & Leinwand, LA 1982, 'Mapping class I gene sequences in the major histocompatibility complex', Nature, vol. 298, no. 5872, pp. 382-385.
Pease LR, Nathenson SG, Leinwand LA. Mapping class I gene sequences in the major histocompatibility complex. Nature. 1982;298(5872):382-385.
Pease, Larry R ; Nathenson, S. G. ; Leinwand, L. A. / Mapping class I gene sequences in the major histocompatibility complex. In: Nature. 1982 ; Vol. 298, No. 5872. pp. 382-385.
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