Mammalian germ-line transgenesis by transposition

Adam J. Dupuy, Karl Clark, Corey M. Carlson, Sabine Fritz, Ann E. Davidson, Karra M. Markley, Ken Finley, Colin F. Fletcher, Stephen C. Ekker, Perry B. Hackett, Sandra Horn, David A. Largaespada

Research output: Contribution to journalArticlepeer-review

170 Scopus citations

Abstract

Transposons have been used in invertebrates for transgenesis and insertional mutagens in genetic screens. We tested a functional transposon called Sleeping Beauty in the one-cell mouse embryo. In this report, we describe experiments in which transposon vectors were injected into one-cell mouse embryos with mRNA expressing the SB10 transposase enzyme. Molecular evidence of transposition was obtained by cloning of insertion sites from multiple transgenic mice produced by SB10 mRNA/transposon coinjection. We also demonstrate germ-line transmission and expression from transposed elements. This technique has promise as a germ-line transgenesis method in other vertebrate species and for insertional mutagenesis in the mouse.

Original languageEnglish (US)
Pages (from-to)4495-4499
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number7
DOIs
StatePublished - Apr 2 2002

Keywords

  • Insertional mutagenesis
  • Sleeping Beauty
  • Transposon

ASJC Scopus subject areas

  • General

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