MALDI imaging mass spectrometry profiling of proteins and lipids in clear cell renal cell carcinoma

Elizabeth Ellen Jones, Thomas W. Powers, Benjamin A. Neely, Lisa H. Cazares, Dean A. Troyer, Alexander Parker, Richard R. Drake

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Reducing the incidence and mortality rates for clear cell renal cell carcinoma (ccRCC) remains a significant clinical challenge with poor 5-year survival rates. A unique tissue cohort was assembled of matched ccRCC and distal nontumor tissues (n = 20) associated with moderate risk of disease progression, half of these from individuals who progressed to metastatic disease and the other half who remained disease free. These tissues were used for MALDI imaging MS profiling of proteins in the 2-20 kDa range, resulting in a panel of 108 proteins that had potential disease-specific expression patterns. Protein lysates from the same tissues were analyzed by MS/MS, resulting in identification of 56 proteins of less than 20 kDa molecular weight. The same tissues were also used for global lipid profiling analysis by MALDI-FT-ICR MS. From the cumulative protein and lipid expression profile data, a refined panel of 26 proteins and 39 lipid species was identified that could either distinguish tumor from nontumor tissues, or tissues from recurrent disease progressors from nonrecurrent disease individuals. This approach has the potential to not only improve prognostic assessment and enhance postoperative surveillance, but also to inform on the underlying biology of ccRCC progression.

Original languageEnglish (US)
Pages (from-to)924-935
Number of pages12
JournalProteomics
Volume14
Issue number7-8
DOIs
StatePublished - 2014

Fingerprint

Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Renal Cell Carcinoma
Mass spectrometry
Mass Spectrometry
Cells
Tissue
Lipids
Imaging techniques
Proteins
Disease Progression
Tumors
Survival Rate
Molecular Weight
Molecular weight
Mortality
Incidence
Neoplasms

Keywords

  • Biomedicine
  • Lipid profiling
  • MALDI imaging
  • MALDI-FT-ICR
  • Renal cancer
  • Tissue proteomics

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry
  • Medicine(all)

Cite this

Jones, E. E., Powers, T. W., Neely, B. A., Cazares, L. H., Troyer, D. A., Parker, A., & Drake, R. R. (2014). MALDI imaging mass spectrometry profiling of proteins and lipids in clear cell renal cell carcinoma. Proteomics, 14(7-8), 924-935. https://doi.org/10.1002/pmic.201300434

MALDI imaging mass spectrometry profiling of proteins and lipids in clear cell renal cell carcinoma. / Jones, Elizabeth Ellen; Powers, Thomas W.; Neely, Benjamin A.; Cazares, Lisa H.; Troyer, Dean A.; Parker, Alexander; Drake, Richard R.

In: Proteomics, Vol. 14, No. 7-8, 2014, p. 924-935.

Research output: Contribution to journalArticle

Jones, EE, Powers, TW, Neely, BA, Cazares, LH, Troyer, DA, Parker, A & Drake, RR 2014, 'MALDI imaging mass spectrometry profiling of proteins and lipids in clear cell renal cell carcinoma', Proteomics, vol. 14, no. 7-8, pp. 924-935. https://doi.org/10.1002/pmic.201300434
Jones, Elizabeth Ellen ; Powers, Thomas W. ; Neely, Benjamin A. ; Cazares, Lisa H. ; Troyer, Dean A. ; Parker, Alexander ; Drake, Richard R. / MALDI imaging mass spectrometry profiling of proteins and lipids in clear cell renal cell carcinoma. In: Proteomics. 2014 ; Vol. 14, No. 7-8. pp. 924-935.
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