Magnetic forces enable rapid endothelialization of synthetic vascular grafts

Sorin V. Pislaru, Adriana Harbuzariu, Gautam Agarwal, Tyra Witt, Rajiv Gulati, Nicole P. Sandhu, Cheryl Mueske, Manju Kalra, Robert D. Simari, Gurpreet S Sandhu

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

BACKGROUND - Synthetic vascular grafts cannot be used in small vessels because of graft failure caused by thrombosis and neointima formation. Rapid endothelialization may overcome this limitation. We hypothesized that a magnetic graft would be able to capture and retain endothelial cells labeled with paramagnetic particles. METHODS AND RESULTS - Porcine blood derived endothelial cells were allowed to endocytose superparamagnetic iron oxide microspheres. Cell survival was assessed by trypan blue exclusion and demonstrated a dose-dependent cell survival of 75% to 95%. A flexible magnetic sheet was annealed to the external surface of a knitted Dacron graft. Labeled cells (10/mL) were placed within the graft for 5 minutes. Confocal and electron microscopy confirmed uniform cell capture at the magnetized surface. The effect of shear forces on the adherent cells was evaluated in a flow chamber. The cells remained attached at rates up to 300 mL/min, with cell loss commencing at 400 mL/min. Prototype magnetic grafts were implanted in porcine carotid arteries. Labeled cells were placed within the graft for 10 minutes at the time of implantation. The grafts were evaluated after one day and uniform cell coverage was noted on the magnetized surface. In comparison, relatively few labeled cells were seen attached to a nonmagnetized surface. CONCLUSIONS - Magnetic forces can be used to rapidly cover a vascular graft with paramagnetically labeled cells. This biophysical interaction is sufficient to retain cells in the presence of blood flow. Applications of this technique may include rapid endothelialization of synthetic vascular grafts and dialysis fistulas.

Original languageEnglish (US)
JournalCirculation
Volume114
Issue numberSUPPL. 1
DOIs
StatePublished - Jul 2006

Fingerprint

Blood Vessels
Transplants
Cell Survival
Swine
Endothelial Cells
Neointima
Polyethylene Terephthalates
Trypan Blue
Endocytosis
Microspheres
Carotid Arteries
Confocal Microscopy
Fistula
Dialysis
Electron Microscopy
Thrombosis

Keywords

  • Coronary disease
  • Endothelium
  • Grafting
  • Magnet
  • Surgery

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Pislaru, S. V., Harbuzariu, A., Agarwal, G., Witt, T., Gulati, R., Sandhu, N. P., ... Sandhu, G. S. (2006). Magnetic forces enable rapid endothelialization of synthetic vascular grafts. Circulation, 114(SUPPL. 1). https://doi.org/10.1161/CIRCULATIONAHA.105.001446

Magnetic forces enable rapid endothelialization of synthetic vascular grafts. / Pislaru, Sorin V.; Harbuzariu, Adriana; Agarwal, Gautam; Witt, Tyra; Gulati, Rajiv; Sandhu, Nicole P.; Mueske, Cheryl; Kalra, Manju; Simari, Robert D.; Sandhu, Gurpreet S.

In: Circulation, Vol. 114, No. SUPPL. 1, 07.2006.

Research output: Contribution to journalArticle

Pislaru, SV, Harbuzariu, A, Agarwal, G, Witt, T, Gulati, R, Sandhu, NP, Mueske, C, Kalra, M, Simari, RD & Sandhu, GS 2006, 'Magnetic forces enable rapid endothelialization of synthetic vascular grafts', Circulation, vol. 114, no. SUPPL. 1. https://doi.org/10.1161/CIRCULATIONAHA.105.001446
Pislaru, Sorin V. ; Harbuzariu, Adriana ; Agarwal, Gautam ; Witt, Tyra ; Gulati, Rajiv ; Sandhu, Nicole P. ; Mueske, Cheryl ; Kalra, Manju ; Simari, Robert D. ; Sandhu, Gurpreet S. / Magnetic forces enable rapid endothelialization of synthetic vascular grafts. In: Circulation. 2006 ; Vol. 114, No. SUPPL. 1.
@article{a6d91a6ea2434369b79ab8c20e9a68fb,
title = "Magnetic forces enable rapid endothelialization of synthetic vascular grafts",
abstract = "BACKGROUND - Synthetic vascular grafts cannot be used in small vessels because of graft failure caused by thrombosis and neointima formation. Rapid endothelialization may overcome this limitation. We hypothesized that a magnetic graft would be able to capture and retain endothelial cells labeled with paramagnetic particles. METHODS AND RESULTS - Porcine blood derived endothelial cells were allowed to endocytose superparamagnetic iron oxide microspheres. Cell survival was assessed by trypan blue exclusion and demonstrated a dose-dependent cell survival of 75{\%} to 95{\%}. A flexible magnetic sheet was annealed to the external surface of a knitted Dacron graft. Labeled cells (10/mL) were placed within the graft for 5 minutes. Confocal and electron microscopy confirmed uniform cell capture at the magnetized surface. The effect of shear forces on the adherent cells was evaluated in a flow chamber. The cells remained attached at rates up to 300 mL/min, with cell loss commencing at 400 mL/min. Prototype magnetic grafts were implanted in porcine carotid arteries. Labeled cells were placed within the graft for 10 minutes at the time of implantation. The grafts were evaluated after one day and uniform cell coverage was noted on the magnetized surface. In comparison, relatively few labeled cells were seen attached to a nonmagnetized surface. CONCLUSIONS - Magnetic forces can be used to rapidly cover a vascular graft with paramagnetically labeled cells. This biophysical interaction is sufficient to retain cells in the presence of blood flow. Applications of this technique may include rapid endothelialization of synthetic vascular grafts and dialysis fistulas.",
keywords = "Coronary disease, Endothelium, Grafting, Magnet, Surgery",
author = "Pislaru, {Sorin V.} and Adriana Harbuzariu and Gautam Agarwal and Tyra Witt and Rajiv Gulati and Sandhu, {Nicole P.} and Cheryl Mueske and Manju Kalra and Simari, {Robert D.} and Sandhu, {Gurpreet S}",
year = "2006",
month = "7",
doi = "10.1161/CIRCULATIONAHA.105.001446",
language = "English (US)",
volume = "114",
journal = "Circulation",
issn = "0009-7322",
publisher = "Lippincott Williams and Wilkins",
number = "SUPPL. 1",

}

TY - JOUR

T1 - Magnetic forces enable rapid endothelialization of synthetic vascular grafts

AU - Pislaru, Sorin V.

AU - Harbuzariu, Adriana

AU - Agarwal, Gautam

AU - Witt, Tyra

AU - Gulati, Rajiv

AU - Sandhu, Nicole P.

AU - Mueske, Cheryl

AU - Kalra, Manju

AU - Simari, Robert D.

AU - Sandhu, Gurpreet S

PY - 2006/7

Y1 - 2006/7

N2 - BACKGROUND - Synthetic vascular grafts cannot be used in small vessels because of graft failure caused by thrombosis and neointima formation. Rapid endothelialization may overcome this limitation. We hypothesized that a magnetic graft would be able to capture and retain endothelial cells labeled with paramagnetic particles. METHODS AND RESULTS - Porcine blood derived endothelial cells were allowed to endocytose superparamagnetic iron oxide microspheres. Cell survival was assessed by trypan blue exclusion and demonstrated a dose-dependent cell survival of 75% to 95%. A flexible magnetic sheet was annealed to the external surface of a knitted Dacron graft. Labeled cells (10/mL) were placed within the graft for 5 minutes. Confocal and electron microscopy confirmed uniform cell capture at the magnetized surface. The effect of shear forces on the adherent cells was evaluated in a flow chamber. The cells remained attached at rates up to 300 mL/min, with cell loss commencing at 400 mL/min. Prototype magnetic grafts were implanted in porcine carotid arteries. Labeled cells were placed within the graft for 10 minutes at the time of implantation. The grafts were evaluated after one day and uniform cell coverage was noted on the magnetized surface. In comparison, relatively few labeled cells were seen attached to a nonmagnetized surface. CONCLUSIONS - Magnetic forces can be used to rapidly cover a vascular graft with paramagnetically labeled cells. This biophysical interaction is sufficient to retain cells in the presence of blood flow. Applications of this technique may include rapid endothelialization of synthetic vascular grafts and dialysis fistulas.

AB - BACKGROUND - Synthetic vascular grafts cannot be used in small vessels because of graft failure caused by thrombosis and neointima formation. Rapid endothelialization may overcome this limitation. We hypothesized that a magnetic graft would be able to capture and retain endothelial cells labeled with paramagnetic particles. METHODS AND RESULTS - Porcine blood derived endothelial cells were allowed to endocytose superparamagnetic iron oxide microspheres. Cell survival was assessed by trypan blue exclusion and demonstrated a dose-dependent cell survival of 75% to 95%. A flexible magnetic sheet was annealed to the external surface of a knitted Dacron graft. Labeled cells (10/mL) were placed within the graft for 5 minutes. Confocal and electron microscopy confirmed uniform cell capture at the magnetized surface. The effect of shear forces on the adherent cells was evaluated in a flow chamber. The cells remained attached at rates up to 300 mL/min, with cell loss commencing at 400 mL/min. Prototype magnetic grafts were implanted in porcine carotid arteries. Labeled cells were placed within the graft for 10 minutes at the time of implantation. The grafts were evaluated after one day and uniform cell coverage was noted on the magnetized surface. In comparison, relatively few labeled cells were seen attached to a nonmagnetized surface. CONCLUSIONS - Magnetic forces can be used to rapidly cover a vascular graft with paramagnetically labeled cells. This biophysical interaction is sufficient to retain cells in the presence of blood flow. Applications of this technique may include rapid endothelialization of synthetic vascular grafts and dialysis fistulas.

KW - Coronary disease

KW - Endothelium

KW - Grafting

KW - Magnet

KW - Surgery

UR - http://www.scopus.com/inward/record.url?scp=33747187002&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33747187002&partnerID=8YFLogxK

U2 - 10.1161/CIRCULATIONAHA.105.001446

DO - 10.1161/CIRCULATIONAHA.105.001446

M3 - Article

C2 - 16820592

AN - SCOPUS:33747187002

VL - 114

JO - Circulation

JF - Circulation

SN - 0009-7322

IS - SUPPL. 1

ER -