M-protein in chicken cardiac muscle

E. E. Strehler; G. Pelloni; C. W. Heizmann; H. M. Eppenberger

doi:10.1016/0014-4827(79)90255-6

M-protein in chicken cardiac muscle

E. E. Strehler, G. Pelloni, C. W. Heizmann, H. M. Eppenberger

Biochemistry and Molecular Biology

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Chicken cardiac muscle myofibrils lack a visible M-line. Antibodies against chicken breast muscle M-protein, an M-line component with M_r = 165 000, were used to demonstrate the presence of a similar protein in chicken heart muscle. The immunoreplica technique showed the heart protein to have about the same molecular weight as the breast muscle M-protein on polyacrylamide slab gels in the presence of sodium dodecyl sulfate (SDS). Positive staining within the H-zone was observed when the indirect immunofluorescence technique was used to localize the M-protein in isolated heart myofibrils. This result was confirmed by electron microscopic investigations on longitudinal sections of antibody-incubated heart muscle fiber bundles showing the antibody against M-protein to be bound within a region corresponding to the M-line region of breast muscle myofibrils.

Original language	English (US)
Pages (from-to)	39-45
Number of pages	7
Journal	Experimental Cell Research
Volume	124
Issue number	1
DOIs	https://doi.org/10.1016/0014-4827(79)90255-6
State	Published - Nov 1979

ASJC Scopus subject areas

Cell Biology

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10.1016/0014-4827(79)90255-6

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title = "M-protein in chicken cardiac muscle",

abstract = "Chicken cardiac muscle myofibrils lack a visible M-line. Antibodies against chicken breast muscle M-protein, an M-line component with Mr = 165 000, were used to demonstrate the presence of a similar protein in chicken heart muscle. The immunoreplica technique showed the heart protein to have about the same molecular weight as the breast muscle M-protein on polyacrylamide slab gels in the presence of sodium dodecyl sulfate (SDS). Positive staining within the H-zone was observed when the indirect immunofluorescence technique was used to localize the M-protein in isolated heart myofibrils. This result was confirmed by electron microscopic investigations on longitudinal sections of antibody-incubated heart muscle fiber bundles showing the antibody against M-protein to be bound within a region corresponding to the M-line region of breast muscle myofibrils.",

author = "Strehler, {E. E.} and G. Pelloni and Heizmann, {C. W.} and Eppenberger, {H. M.}",

note = "Funding Information: the Swiss National Science Foundation and by a grant to H.M.E. from the Muscular Dystrophy Association,",

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T1 - M-protein in chicken cardiac muscle

AU - Strehler, E. E.

AU - Pelloni, G.

AU - Heizmann, C. W.

AU - Eppenberger, H. M.

N1 - Funding Information: the Swiss National Science Foundation and by a grant to H.M.E. from the Muscular Dystrophy Association,

PY - 1979/11

Y1 - 1979/11

N2 - Chicken cardiac muscle myofibrils lack a visible M-line. Antibodies against chicken breast muscle M-protein, an M-line component with Mr = 165 000, were used to demonstrate the presence of a similar protein in chicken heart muscle. The immunoreplica technique showed the heart protein to have about the same molecular weight as the breast muscle M-protein on polyacrylamide slab gels in the presence of sodium dodecyl sulfate (SDS). Positive staining within the H-zone was observed when the indirect immunofluorescence technique was used to localize the M-protein in isolated heart myofibrils. This result was confirmed by electron microscopic investigations on longitudinal sections of antibody-incubated heart muscle fiber bundles showing the antibody against M-protein to be bound within a region corresponding to the M-line region of breast muscle myofibrils.

AB - Chicken cardiac muscle myofibrils lack a visible M-line. Antibodies against chicken breast muscle M-protein, an M-line component with Mr = 165 000, were used to demonstrate the presence of a similar protein in chicken heart muscle. The immunoreplica technique showed the heart protein to have about the same molecular weight as the breast muscle M-protein on polyacrylamide slab gels in the presence of sodium dodecyl sulfate (SDS). Positive staining within the H-zone was observed when the indirect immunofluorescence technique was used to localize the M-protein in isolated heart myofibrils. This result was confirmed by electron microscopic investigations on longitudinal sections of antibody-incubated heart muscle fiber bundles showing the antibody against M-protein to be bound within a region corresponding to the M-line region of breast muscle myofibrils.

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M-protein in chicken cardiac muscle

Abstract

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