M-protein in chicken cardiac muscle

E. E. Strehler, G. Pelloni, C. W. Heizmann, H. M. Eppenberger

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Chicken cardiac muscle myofibrils lack a visible M-line. Antibodies against chicken breast muscle M-protein, an M-line component with Mr = 165 000, were used to demonstrate the presence of a similar protein in chicken heart muscle. The immunoreplica technique showed the heart protein to have about the same molecular weight as the breast muscle M-protein on polyacrylamide slab gels in the presence of sodium dodecyl sulfate (SDS). Positive staining within the H-zone was observed when the indirect immunofluorescence technique was used to localize the M-protein in isolated heart myofibrils. This result was confirmed by electron microscopic investigations on longitudinal sections of antibody-incubated heart muscle fiber bundles showing the antibody against M-protein to be bound within a region corresponding to the M-line region of breast muscle myofibrils.

Original languageEnglish (US)
Pages (from-to)39-45
Number of pages7
JournalExperimental Cell Research
Volume124
Issue number1
DOIs
StatePublished - Nov 1979

ASJC Scopus subject areas

  • Cell Biology

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    Strehler, E. E., Pelloni, G., Heizmann, C. W., & Eppenberger, H. M. (1979). M-protein in chicken cardiac muscle. Experimental Cell Research, 124(1), 39-45. https://doi.org/10.1016/0014-4827(79)90255-6