Lysophospholipids increase interleukin-8 expression in ovarian cancer cells

Benjamin M. Schwartz, Guiying Hong, Bei H. Morrison, Weihua Wu, Linnea M. Baudhuin, Yi Jin Xiao, Samuel C. Mok, Yan Xu

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

Objectives. We have previously described that bioactive lysophospholipids - lysophosphatidic acid (LPA), sphingosine 1-phosphate (SIP), and sphingosylphosphorylcholine (SPC) - are present in ascitic fluids from patients with ovarian cancer. To understand the role of these lipids in ovarian cancer, we investigated the effects of these lipids on interleukin-8 (IL-8) production in ovarian cancer cells. IL-8 is a proinflammatory and proangiogenic factor, which is potentially involved in ovarian cancer development. Methods. The Clontech PCR-Select cDNA subtraction method (Clontech Laboratories, Inc., Palo Alto, CA) was used to identify genes potentially regulated by LPA in HEY and OCC1 ovarian cancer cell lines. Northern blot analysis was used to confirm and examine IL-8 mRNA regulation by lysolipids. Enzyme-linked immunosorbent assay (ELISA) was used for detecting secreted IL-8. Results. We describe here that LPA, S1P, and SPC increased mRNA levels (2- to 7-fold) and protein secretion (2- to 12-fold) of IL-8 from ovarian cancer cells (HEY, OCC1, and SKOV3) in vitro. These regulations were both dose- and time-dependent. All three lipids increased the stability IL-8 mRNA in HEY cells. In contrast to malignant ovarian cancer cells, immortalized human ovarian epithelial cells did not respond to any of these lipids to increase the secretion of IL-8, although these cells secreted similar basal levels of IL-8 (310 pg/ml/10,000 cells). Two breast cancer cell lines (MCF7 and T47D) secreted lower basal levels of IL-8 (48-80 pg/ml/10,000 cells), compared with ovarian cancer cells (200-500 pg/ml/10,000 cells). MCF7 cells responded to LPA, but not S1P and SPC, by increasing the secretion of IL-8. T47D and MCF10A, an immortalized breast cell line, did not respond to LPA, S1P, or SPC to increase IL-8 secretion. Conclusions. LPA, S1P, and SPC regulate the mRNA and protein levels of the proinflammatory and proangiogenic factor IL-8 in ovarian cancer cells. The pathological significance of these regulations in ovarian cancer remains to be further investigated.

Original languageEnglish (US)
Pages (from-to)291-300
Number of pages10
JournalGynecologic Oncology
Volume81
Issue number2
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Lysophospholipids
Interleukin-8
Ovarian Neoplasms
Lipids
Messenger RNA
Cell Line
Ascitic Fluid
MCF-7 Cells
Northern Blotting
lysophosphatidic acid

Keywords

  • Interleukin-8
  • Lysophosphatidic acid
  • Ovarian cancer
  • Sphingosine 1-phosphate
  • Sphingosylphosphorylcholine (SPC)

ASJC Scopus subject areas

  • Medicine(all)
  • Oncology
  • Obstetrics and Gynecology

Cite this

Schwartz, B. M., Hong, G., Morrison, B. H., Wu, W., Baudhuin, L. M., Xiao, Y. J., ... Xu, Y. (2001). Lysophospholipids increase interleukin-8 expression in ovarian cancer cells. Gynecologic Oncology, 81(2), 291-300. https://doi.org/10.1006/gyno.2001.6124

Lysophospholipids increase interleukin-8 expression in ovarian cancer cells. / Schwartz, Benjamin M.; Hong, Guiying; Morrison, Bei H.; Wu, Weihua; Baudhuin, Linnea M.; Xiao, Yi Jin; Mok, Samuel C.; Xu, Yan.

In: Gynecologic Oncology, Vol. 81, No. 2, 2001, p. 291-300.

Research output: Contribution to journalArticle

Schwartz, BM, Hong, G, Morrison, BH, Wu, W, Baudhuin, LM, Xiao, YJ, Mok, SC & Xu, Y 2001, 'Lysophospholipids increase interleukin-8 expression in ovarian cancer cells', Gynecologic Oncology, vol. 81, no. 2, pp. 291-300. https://doi.org/10.1006/gyno.2001.6124
Schwartz BM, Hong G, Morrison BH, Wu W, Baudhuin LM, Xiao YJ et al. Lysophospholipids increase interleukin-8 expression in ovarian cancer cells. Gynecologic Oncology. 2001;81(2):291-300. https://doi.org/10.1006/gyno.2001.6124
Schwartz, Benjamin M. ; Hong, Guiying ; Morrison, Bei H. ; Wu, Weihua ; Baudhuin, Linnea M. ; Xiao, Yi Jin ; Mok, Samuel C. ; Xu, Yan. / Lysophospholipids increase interleukin-8 expression in ovarian cancer cells. In: Gynecologic Oncology. 2001 ; Vol. 81, No. 2. pp. 291-300.
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abstract = "Objectives. We have previously described that bioactive lysophospholipids - lysophosphatidic acid (LPA), sphingosine 1-phosphate (SIP), and sphingosylphosphorylcholine (SPC) - are present in ascitic fluids from patients with ovarian cancer. To understand the role of these lipids in ovarian cancer, we investigated the effects of these lipids on interleukin-8 (IL-8) production in ovarian cancer cells. IL-8 is a proinflammatory and proangiogenic factor, which is potentially involved in ovarian cancer development. Methods. The Clontech PCR-Select cDNA subtraction method (Clontech Laboratories, Inc., Palo Alto, CA) was used to identify genes potentially regulated by LPA in HEY and OCC1 ovarian cancer cell lines. Northern blot analysis was used to confirm and examine IL-8 mRNA regulation by lysolipids. Enzyme-linked immunosorbent assay (ELISA) was used for detecting secreted IL-8. Results. We describe here that LPA, S1P, and SPC increased mRNA levels (2- to 7-fold) and protein secretion (2- to 12-fold) of IL-8 from ovarian cancer cells (HEY, OCC1, and SKOV3) in vitro. These regulations were both dose- and time-dependent. All three lipids increased the stability IL-8 mRNA in HEY cells. In contrast to malignant ovarian cancer cells, immortalized human ovarian epithelial cells did not respond to any of these lipids to increase the secretion of IL-8, although these cells secreted similar basal levels of IL-8 (310 pg/ml/10,000 cells). Two breast cancer cell lines (MCF7 and T47D) secreted lower basal levels of IL-8 (48-80 pg/ml/10,000 cells), compared with ovarian cancer cells (200-500 pg/ml/10,000 cells). MCF7 cells responded to LPA, but not S1P and SPC, by increasing the secretion of IL-8. T47D and MCF10A, an immortalized breast cell line, did not respond to LPA, S1P, or SPC to increase IL-8 secretion. Conclusions. LPA, S1P, and SPC regulate the mRNA and protein levels of the proinflammatory and proangiogenic factor IL-8 in ovarian cancer cells. The pathological significance of these regulations in ovarian cancer remains to be further investigated.",
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AU - Schwartz, Benjamin M.

AU - Hong, Guiying

AU - Morrison, Bei H.

AU - Wu, Weihua

AU - Baudhuin, Linnea M.

AU - Xiao, Yi Jin

AU - Mok, Samuel C.

AU - Xu, Yan

PY - 2001

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N2 - Objectives. We have previously described that bioactive lysophospholipids - lysophosphatidic acid (LPA), sphingosine 1-phosphate (SIP), and sphingosylphosphorylcholine (SPC) - are present in ascitic fluids from patients with ovarian cancer. To understand the role of these lipids in ovarian cancer, we investigated the effects of these lipids on interleukin-8 (IL-8) production in ovarian cancer cells. IL-8 is a proinflammatory and proangiogenic factor, which is potentially involved in ovarian cancer development. Methods. The Clontech PCR-Select cDNA subtraction method (Clontech Laboratories, Inc., Palo Alto, CA) was used to identify genes potentially regulated by LPA in HEY and OCC1 ovarian cancer cell lines. Northern blot analysis was used to confirm and examine IL-8 mRNA regulation by lysolipids. Enzyme-linked immunosorbent assay (ELISA) was used for detecting secreted IL-8. Results. We describe here that LPA, S1P, and SPC increased mRNA levels (2- to 7-fold) and protein secretion (2- to 12-fold) of IL-8 from ovarian cancer cells (HEY, OCC1, and SKOV3) in vitro. These regulations were both dose- and time-dependent. All three lipids increased the stability IL-8 mRNA in HEY cells. In contrast to malignant ovarian cancer cells, immortalized human ovarian epithelial cells did not respond to any of these lipids to increase the secretion of IL-8, although these cells secreted similar basal levels of IL-8 (310 pg/ml/10,000 cells). Two breast cancer cell lines (MCF7 and T47D) secreted lower basal levels of IL-8 (48-80 pg/ml/10,000 cells), compared with ovarian cancer cells (200-500 pg/ml/10,000 cells). MCF7 cells responded to LPA, but not S1P and SPC, by increasing the secretion of IL-8. T47D and MCF10A, an immortalized breast cell line, did not respond to LPA, S1P, or SPC to increase IL-8 secretion. Conclusions. LPA, S1P, and SPC regulate the mRNA and protein levels of the proinflammatory and proangiogenic factor IL-8 in ovarian cancer cells. The pathological significance of these regulations in ovarian cancer remains to be further investigated.

AB - Objectives. We have previously described that bioactive lysophospholipids - lysophosphatidic acid (LPA), sphingosine 1-phosphate (SIP), and sphingosylphosphorylcholine (SPC) - are present in ascitic fluids from patients with ovarian cancer. To understand the role of these lipids in ovarian cancer, we investigated the effects of these lipids on interleukin-8 (IL-8) production in ovarian cancer cells. IL-8 is a proinflammatory and proangiogenic factor, which is potentially involved in ovarian cancer development. Methods. The Clontech PCR-Select cDNA subtraction method (Clontech Laboratories, Inc., Palo Alto, CA) was used to identify genes potentially regulated by LPA in HEY and OCC1 ovarian cancer cell lines. Northern blot analysis was used to confirm and examine IL-8 mRNA regulation by lysolipids. Enzyme-linked immunosorbent assay (ELISA) was used for detecting secreted IL-8. Results. We describe here that LPA, S1P, and SPC increased mRNA levels (2- to 7-fold) and protein secretion (2- to 12-fold) of IL-8 from ovarian cancer cells (HEY, OCC1, and SKOV3) in vitro. These regulations were both dose- and time-dependent. All three lipids increased the stability IL-8 mRNA in HEY cells. In contrast to malignant ovarian cancer cells, immortalized human ovarian epithelial cells did not respond to any of these lipids to increase the secretion of IL-8, although these cells secreted similar basal levels of IL-8 (310 pg/ml/10,000 cells). Two breast cancer cell lines (MCF7 and T47D) secreted lower basal levels of IL-8 (48-80 pg/ml/10,000 cells), compared with ovarian cancer cells (200-500 pg/ml/10,000 cells). MCF7 cells responded to LPA, but not S1P and SPC, by increasing the secretion of IL-8. T47D and MCF10A, an immortalized breast cell line, did not respond to LPA, S1P, or SPC to increase IL-8 secretion. Conclusions. LPA, S1P, and SPC regulate the mRNA and protein levels of the proinflammatory and proangiogenic factor IL-8 in ovarian cancer cells. The pathological significance of these regulations in ovarian cancer remains to be further investigated.

KW - Interleukin-8

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KW - Sphingosine 1-phosphate

KW - Sphingosylphosphorylcholine (SPC)

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