Luteinizing hormone stimulates the production of prostacyclin by isolated ovarian cell in vitro

Johannes D. Veldhuis; Patricia Klase; Laurence M. Demers

doi:10.1016/0090-6980(82)90077-6

Luteinizing hormone stimulates the production of prostacyclin by isolated ovarian cell in vitro

Johannes D. Veldhuis, Patricia Klase, Laurence M. Demers

Endocrinology, Diabetes, Metabolism, and Nutrition

Research output: Contribution to journal › Article

4 Scopus citations

Abstract

Granulosa cells isolated from mature Graafian follicles of swine produced significant quantities of immunoreactive 6-keto-PGF₁α under chemically defined conditions in vitro. Luteinizing hormone elicited a dose-dependent stimulation of 6-keto-PGF₁α accumulation, but follicle stimulating hormone, prolactin, L-epinephrine, estradiol-17B, or PGE₂ were devoid of effect. The time-dependent in vitro production of 6-keto-PGF₁α by ovarian cells was susceptible to inhibition by indomethacin, U-51506, cycloheximide, and actinomycin D. These observations implicate granulosa cells in the specific and hormonally regulated production of prostacyclin.

Original language	English (US)
Pages (from-to)	319-327
Number of pages	9
Journal	Prostaglandins
Volume	23
Issue number	3
DOIs	https://doi.org/10.1016/0090-6980(82)90077-6
State	Published - Mar 1982

ASJC Scopus subject areas

Biochemistry
Endocrinology

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Veldhuis, J. D., Klase, P., & Demers, L. M. (1982). Luteinizing hormone stimulates the production of prostacyclin by isolated ovarian cell in vitro. Prostaglandins, 23(3), 319-327. https://doi.org/10.1016/0090-6980(82)90077-6

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N2 - Granulosa cells isolated from mature Graafian follicles of swine produced significant quantities of immunoreactive 6-keto-PGF1α under chemically defined conditions in vitro. Luteinizing hormone elicited a dose-dependent stimulation of 6-keto-PGF1α accumulation, but follicle stimulating hormone, prolactin, L-epinephrine, estradiol-17B, or PGE2 were devoid of effect. The time-dependent in vitro production of 6-keto-PGF1α by ovarian cells was susceptible to inhibition by indomethacin, U-51506, cycloheximide, and actinomycin D. These observations implicate granulosa cells in the specific and hormonally regulated production of prostacyclin.

AB - Granulosa cells isolated from mature Graafian follicles of swine produced significant quantities of immunoreactive 6-keto-PGF1α under chemically defined conditions in vitro. Luteinizing hormone elicited a dose-dependent stimulation of 6-keto-PGF1α accumulation, but follicle stimulating hormone, prolactin, L-epinephrine, estradiol-17B, or PGE2 were devoid of effect. The time-dependent in vitro production of 6-keto-PGF1α by ovarian cells was susceptible to inhibition by indomethacin, U-51506, cycloheximide, and actinomycin D. These observations implicate granulosa cells in the specific and hormonally regulated production of prostacyclin.

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