TY - JOUR
T1 - Luteinizing hormone stimulates the production of prostacyclin by isolated ovarian cell in vitro
AU - Veldhuis, Johannes D.
AU - Klase, Patricia
AU - Demers, Laurence M.
N1 - Funding Information:
We appreciate the provision of polypeptide hormones by the NIAMDD, NIH, Bethesda, MD; the gift of U-51605 from Dr. G.L. Bundy (Upjohn Co., Kalamazoo, MI); and the donation of swine ovaries by Arbogast and Bastian, Inc., Allentown, PA. Dennis Derek kindly supervised the radioirmnunoassays. We thank Janet Moore for excellent secretarial assistance. This work was supported in part by a National Research Service Award (to JDV) lF32 HD05755 from the National Institute of Child Health and Human Development, NIH, Bethesda, MD. This work was presented in part at the annual meeting of the Clinical Biochemistry Society, Kansas City, MO, July 1981.
PY - 1982/3
Y1 - 1982/3
N2 - Granulosa cells isolated from mature Graafian follicles of swine produced significant quantities of immunoreactive 6-keto-PGF1α under chemically defined conditions in vitro. Luteinizing hormone elicited a dose-dependent stimulation of 6-keto-PGF1α accumulation, but follicle stimulating hormone, prolactin, L-epinephrine, estradiol-17B, or PGE2 were devoid of effect. The time-dependent in vitro production of 6-keto-PGF1α by ovarian cells was susceptible to inhibition by indomethacin, U-51506, cycloheximide, and actinomycin D. These observations implicate granulosa cells in the specific and hormonally regulated production of prostacyclin.
AB - Granulosa cells isolated from mature Graafian follicles of swine produced significant quantities of immunoreactive 6-keto-PGF1α under chemically defined conditions in vitro. Luteinizing hormone elicited a dose-dependent stimulation of 6-keto-PGF1α accumulation, but follicle stimulating hormone, prolactin, L-epinephrine, estradiol-17B, or PGE2 were devoid of effect. The time-dependent in vitro production of 6-keto-PGF1α by ovarian cells was susceptible to inhibition by indomethacin, U-51506, cycloheximide, and actinomycin D. These observations implicate granulosa cells in the specific and hormonally regulated production of prostacyclin.
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U2 - 10.1016/0090-6980(82)90077-6
DO - 10.1016/0090-6980(82)90077-6
M3 - Article
C2 - 7048424
AN - SCOPUS:0019981582
VL - 23
SP - 319
EP - 327
JO - Prostaglandins
JF - Prostaglandins
SN - 0090-6980
IS - 3
ER -