TY - JOUR
T1 - Luteinizing hormone stimulates the production of prostacyclin by isolated ovarian cell in vitro
AU - Veldhuis, Johannes D.
AU - Klase, Patricia
AU - Demers, Laurence M.
PY - 1982/3
Y1 - 1982/3
N2 - Granulosa cells isolated from mature Graafian follicles of swine produced significant quantities of immunoreactive 6-keto-PGF1α under chemically defined conditions in vitro. Luteinizing hormone elicited a dose-dependent stimulation of 6-keto-PGF1α accumulation, but follicle stimulating hormone, prolactin, L-epinephrine, estradiol-17B, or PGE2 were devoid of effect. The time-dependent in vitro production of 6-keto-PGF1α by ovarian cells was susceptible to inhibition by indomethacin, U-51506, cycloheximide, and actinomycin D. These observations implicate granulosa cells in the specific and hormonally regulated production of prostacyclin.
AB - Granulosa cells isolated from mature Graafian follicles of swine produced significant quantities of immunoreactive 6-keto-PGF1α under chemically defined conditions in vitro. Luteinizing hormone elicited a dose-dependent stimulation of 6-keto-PGF1α accumulation, but follicle stimulating hormone, prolactin, L-epinephrine, estradiol-17B, or PGE2 were devoid of effect. The time-dependent in vitro production of 6-keto-PGF1α by ovarian cells was susceptible to inhibition by indomethacin, U-51506, cycloheximide, and actinomycin D. These observations implicate granulosa cells in the specific and hormonally regulated production of prostacyclin.
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U2 - 10.1016/0090-6980(82)90077-6
DO - 10.1016/0090-6980(82)90077-6
M3 - Article
C2 - 7048424
AN - SCOPUS:0019981582
VL - 23
SP - 319
EP - 327
JO - Prostaglandins
JF - Prostaglandins
SN - 0090-6980
IS - 3
ER -