Low density lipoprotein receptor-related protein mediates apolipoprotein E inhibition of smooth muscle cell migration

Debi K. Swertfeger, Guojun D Bu, David Y. Hui

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

This research was undertaken to identify the cell surface receptor responsible for mediating apolipoprotein E (apoE) inhibition of platelet-derived growth factor (PDGF)-directed smooth muscle cell migration. Initial studies revealed the expression of the low density lipoprotein receptor (LDLR), the LDL receptor-related protein (LRP), the very low density lipoprotein receptor (VLDL), and apoE receptor-2 in mouse aortic smooth muscle cells. Smooth muscle cells isolated from LDLR-null, VLDL-null, and apoE receptor-2-null mice were responsive to apoE inhibition of PDGF-directed smooth muscle cell migration, suggesting that these receptors were not involved. An antisense RNA expression knock-down strategy, utilizing morpholino antisense RNA against LRP, was used to reduce LRP expression in smooth muscle cells to assess the role of this receptor in apoE inhibition of cell migration. Results showed that apoE was unable to inhibit PDGF-directed migration of LRP-deficient smooth muscle cells. The role of LRP in mediating apoE inhibition of PDGF-directed smooth muscle cell migration was confirmed by experiments showing that antibodies against LRP effectively suppressed apoE inhibition of PDGF-directed smooth muscle cell migration. Taken together, these results document that apoE binding to LRP is required for its inhibition of PDGF-directed smooth muscle cell migration.

Original languageEnglish (US)
Pages (from-to)4141-4146
Number of pages6
JournalJournal of Biological Chemistry
Volume277
Issue number6
DOIs
StatePublished - Feb 8 2002
Externally publishedYes

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LDL-Receptor Related Proteins
Low Density Lipoprotein Receptor-Related Protein-1
LDL Receptors
Apolipoproteins E
Smooth Muscle Myocytes
Cell Movement
Muscle
Platelet-Derived Growth Factor
Cells
Proteins
Antisense RNA
Cell Migration Inhibition
Morpholinos
Cell Surface Receptors

ASJC Scopus subject areas

  • Biochemistry

Cite this

Low density lipoprotein receptor-related protein mediates apolipoprotein E inhibition of smooth muscle cell migration. / Swertfeger, Debi K.; Bu, Guojun D; Hui, David Y.

In: Journal of Biological Chemistry, Vol. 277, No. 6, 08.02.2002, p. 4141-4146.

Research output: Contribution to journalArticle

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