Abstract
Urinary protein macromolecules have long been thought to play a role in influencing the various phases of urolithiasis including nucleation, growth, aggregation of mineral crystals and their subsequent adhesion to the renal epithelial cells. However, compelling evidence regarding their precise role was lacking, due partly to the fact that most prior studies were done in vitro and results were highly variable depending on the experimental conditions. The advent of genetic engineering technology has made it possible to study urinary protein macromolecules within an in vivo biological system. Indeed, recent studies have begun to shed light on the net effects of loss of one or more macromolecules on the earliest steps of urolithiasis. This paper focuses on the in vivo consequences of inactivating Tamm-Horsfall protein and/or osteopontin, two major urinary glycoproteins, using the knockout approach. The renal phenotypes of both single and double knockout mice under spontaneous or hyperoxaluric conditions will be described. The functional significance of the urinary macromolecules as critical defense factors against renal calcification will also be discussed.
Original language | English (US) |
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Title of host publication | AIP Conference Proceedings |
Pages | 113-119 |
Number of pages | 7 |
Volume | 1049 |
DOIs | |
State | Published - 2008 |
Event | 2nd International Urolithiasis Research Symposium - Indianapolis, IN, United States Duration: Apr 17 2008 → Apr 18 2008 |
Other
Other | 2nd International Urolithiasis Research Symposium |
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Country | United States |
City | Indianapolis, IN |
Period | 4/17/08 → 4/18/08 |
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Keywords
- Kidney stone
- Knockout mice
- Osteopontin
- Tamm-Horsfall protein
- Urinary macromolecules
- Urolithiasis
ASJC Scopus subject areas
- Physics and Astronomy(all)
Cite this
Loss of urinary macromolecules in mice causes interstitial and intratubular renal calcification dependent on the underlying conditions. / Wu, Xue Ru; Lieske, John C; Evan, Andrew P.; Sommer, Andre J.; Liaw, Lucy; Mo, Lan.
AIP Conference Proceedings. Vol. 1049 2008. p. 113-119.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Loss of urinary macromolecules in mice causes interstitial and intratubular renal calcification dependent on the underlying conditions
AU - Wu, Xue Ru
AU - Lieske, John C
AU - Evan, Andrew P.
AU - Sommer, Andre J.
AU - Liaw, Lucy
AU - Mo, Lan
PY - 2008
Y1 - 2008
N2 - Urinary protein macromolecules have long been thought to play a role in influencing the various phases of urolithiasis including nucleation, growth, aggregation of mineral crystals and their subsequent adhesion to the renal epithelial cells. However, compelling evidence regarding their precise role was lacking, due partly to the fact that most prior studies were done in vitro and results were highly variable depending on the experimental conditions. The advent of genetic engineering technology has made it possible to study urinary protein macromolecules within an in vivo biological system. Indeed, recent studies have begun to shed light on the net effects of loss of one or more macromolecules on the earliest steps of urolithiasis. This paper focuses on the in vivo consequences of inactivating Tamm-Horsfall protein and/or osteopontin, two major urinary glycoproteins, using the knockout approach. The renal phenotypes of both single and double knockout mice under spontaneous or hyperoxaluric conditions will be described. The functional significance of the urinary macromolecules as critical defense factors against renal calcification will also be discussed.
AB - Urinary protein macromolecules have long been thought to play a role in influencing the various phases of urolithiasis including nucleation, growth, aggregation of mineral crystals and their subsequent adhesion to the renal epithelial cells. However, compelling evidence regarding their precise role was lacking, due partly to the fact that most prior studies were done in vitro and results were highly variable depending on the experimental conditions. The advent of genetic engineering technology has made it possible to study urinary protein macromolecules within an in vivo biological system. Indeed, recent studies have begun to shed light on the net effects of loss of one or more macromolecules on the earliest steps of urolithiasis. This paper focuses on the in vivo consequences of inactivating Tamm-Horsfall protein and/or osteopontin, two major urinary glycoproteins, using the knockout approach. The renal phenotypes of both single and double knockout mice under spontaneous or hyperoxaluric conditions will be described. The functional significance of the urinary macromolecules as critical defense factors against renal calcification will also be discussed.
KW - Kidney stone
KW - Knockout mice
KW - Osteopontin
KW - Tamm-Horsfall protein
KW - Urinary macromolecules
KW - Urolithiasis
UR - http://www.scopus.com/inward/record.url?scp=54049099625&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=54049099625&partnerID=8YFLogxK
U2 - 10.1063/1.2998004
DO - 10.1063/1.2998004
M3 - Conference contribution
AN - SCOPUS:54049099625
SN - 9780735405776
VL - 1049
SP - 113
EP - 119
BT - AIP Conference Proceedings
ER -