Loss of Immunoreactivity for Human Calmodulin-Like Protein is an Early Event in Breast Cancer Development

Michael S. Rogers, Margaret A. Foley, Thomas B. Crotty, Lynn C. Hartmann, James N. Ingle, Patrick C. Roche, Emanuel E. Strehler

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Cell proliferation requires calmodulin, a protein that regulates calcium-dependent enzymes involved in signal transduction pathways in eukaryotic cells. Calmodulin-like protein (CLP) is found in certain epithelial cell types, including normal breast epithelium, and, although it closely resembles calmodulin in amino acid sequence, CLP interacts with different proteins than does calmodulin. The observation that CLP mRNA expression is dramatically reduced in transformed breast epithelial cells led to two hypotheses: (1) CLP helps to maintain the differentiated state in epithelial cells; and (2) downregulation of CLP accompanies malignant transformation of breast epithelial cells. The objective of this study was to determine if the expression of CLP in human breast cancer specimens is reduced in comparison to its expression in normal breast tissue. Eighty human breast cancer biopsy specimens were analyzed immunohistochemically for CLP expression by using a polyclonal rabbit antihuman CLP antibody. CLP expression was reduced in 79% to 88% of the Invasive ductal carcinoma and lobular carcinoma specimens and in a similar fraction of the ductal carcinoma in-situ specimens, compared with normal breast specimens. None of the breast cancer specimens showed an increase in CLP expression. These findings support the hypotheses that CLP behaves as a functional tumor suppressor protein and is downregulated early in breast cancer progression.

Original languageEnglish (US)
Pages (from-to)220-225
Number of pages6
JournalNeoplasia
Volume1
Issue number3
StatePublished - Aug 1999

Fingerprint

Calmodulin
Breast Neoplasms
Proteins
Breast
Epithelial Cells
human CALML3 protein
Down-Regulation
Tumor Suppressor Proteins
Lobular Carcinoma
Ductal Carcinoma
Carcinoma, Intraductal, Noninfiltrating
Eukaryotic Cells
Amino Acid Sequence
Signal Transduction
Epithelium
Cell Proliferation

Keywords

  • Breast cancer
  • Calmodulin-like protein
  • Epithelial differentiation
  • Immunohistochemistry
  • Tumorigenesis

ASJC Scopus subject areas

  • Cancer Research

Cite this

Rogers, M. S., Foley, M. A., Crotty, T. B., Hartmann, L. C., Ingle, J. N., Roche, P. C., & Strehler, E. E. (1999). Loss of Immunoreactivity for Human Calmodulin-Like Protein is an Early Event in Breast Cancer Development. Neoplasia, 1(3), 220-225.

Loss of Immunoreactivity for Human Calmodulin-Like Protein is an Early Event in Breast Cancer Development. / Rogers, Michael S.; Foley, Margaret A.; Crotty, Thomas B.; Hartmann, Lynn C.; Ingle, James N.; Roche, Patrick C.; Strehler, Emanuel E.

In: Neoplasia, Vol. 1, No. 3, 08.1999, p. 220-225.

Research output: Contribution to journalArticle

Rogers, MS, Foley, MA, Crotty, TB, Hartmann, LC, Ingle, JN, Roche, PC & Strehler, EE 1999, 'Loss of Immunoreactivity for Human Calmodulin-Like Protein is an Early Event in Breast Cancer Development', Neoplasia, vol. 1, no. 3, pp. 220-225.
Rogers MS, Foley MA, Crotty TB, Hartmann LC, Ingle JN, Roche PC et al. Loss of Immunoreactivity for Human Calmodulin-Like Protein is an Early Event in Breast Cancer Development. Neoplasia. 1999 Aug;1(3):220-225.
Rogers, Michael S. ; Foley, Margaret A. ; Crotty, Thomas B. ; Hartmann, Lynn C. ; Ingle, James N. ; Roche, Patrick C. ; Strehler, Emanuel E. / Loss of Immunoreactivity for Human Calmodulin-Like Protein is an Early Event in Breast Cancer Development. In: Neoplasia. 1999 ; Vol. 1, No. 3. pp. 220-225.
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