Loss of estrogen receptor β decreases mitochondrial energetic potential and increases thrombogenicity of platelets in aged female mice

Muthuvel Jayachandran, Claudia C. Preston, Larry W. Hunter, Arshad Jahangir, Whyte G. Owen, Kenneth S. Korach, Virginia M Miller

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Platelets derived from aged (reproductively senescent) female mice with genetic deletion of estrogen receptor beta (βER) are more thrombogenic than those from age-matched wild-type (WT) mice. Intracellular processes contributing to this increased thrombogenicity are not known. Experiments were designed to identify subcellular localization of estrogen receptors and evaluate both glycolytic and mitochondrial energetic processes which might affect platelet activation. Platelets and blood from aged (22-24 months) WT and estrogen receptor β knockout (βERKO) female mice were used in this study. Body, spleen weight, and serum concentrations of follicle-stimulating hormone and 17β-estradiol were comparable between WT and βERKO mice. Number of spontaneous deaths was greater in the βERKO colony (50% compared to 30% in WT) over the course of 24 months. In resting (nonactivated) platelets, estrogen receptors did not appear to colocalize with mitochondria by immunostaining. Lactate production and mitochondrial membrane potential of intact platelets were similar in both groups of mice. However, activities of NADH dehydrogenase, cytochrome bc1 complex, and cytochrome c oxidase of the electron transport chain were reduced in mitochondria isolated from platelets from βERKO compared to WT mice. There were a significantly higher number of phosphatidylserine-expressing platelet-derived microvesicles in the plasma and a greater thrombin-generating capacity in βERKO compared to WT mice. These results suggest that deficiencies in βER affect energy metabolism of platelets resulting in greater production of circulating thrombogenic microvesicles and could potentially explain increased predisposition to thromboembolism in some elderly females.

Original languageEnglish (US)
Pages (from-to)109-121
Number of pages13
JournalAge
Volume32
Issue number1
DOIs
StatePublished - Mar 2010

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Estrogen Receptors
Blood Platelets
Estrogen Receptor beta
Knockout Mice
Mitochondria
NADH Dehydrogenase
Mitochondrial Membrane Potential
Electron Transport Complex III
Phosphatidylserines
Thromboembolism
Platelet Activation
Follicle Stimulating Hormone
Electron Transport Complex IV
Electron Transport
Thrombin
Energy Metabolism
Estradiol
Lactic Acid
Spleen
Body Weight

Keywords

  • Aging
  • Estrogen receptors
  • Microparticles
  • Mitochondria
  • Platelet energy metabolism
  • Procoagulant activity

ASJC Scopus subject areas

  • Aging
  • Geriatrics and Gerontology

Cite this

Loss of estrogen receptor β decreases mitochondrial energetic potential and increases thrombogenicity of platelets in aged female mice. / Jayachandran, Muthuvel; Preston, Claudia C.; Hunter, Larry W.; Jahangir, Arshad; Owen, Whyte G.; Korach, Kenneth S.; Miller, Virginia M.

In: Age, Vol. 32, No. 1, 03.2010, p. 109-121.

Research output: Contribution to journalArticle

Jayachandran, Muthuvel ; Preston, Claudia C. ; Hunter, Larry W. ; Jahangir, Arshad ; Owen, Whyte G. ; Korach, Kenneth S. ; Miller, Virginia M. / Loss of estrogen receptor β decreases mitochondrial energetic potential and increases thrombogenicity of platelets in aged female mice. In: Age. 2010 ; Vol. 32, No. 1. pp. 109-121.
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