Loss of caveolin-1 expression in knock-in mouse model of huntington's disease suppresses pathophysiology in vivo

Eugenia D Trushina, Christie A. Canaria, Do Yup Lee, Cynthia T. McMurray

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Loss of cholesterolhomeostasis andaltered vesicle trafficking have been detected in Huntington's disease (HD) cellular and animalmodels, yet the role of these dysfunctions in pathophysiology ofHDis unknown.Wedemonstrate here that defects in caveolar-related cholesterol trafficking directly contribute to the mechanism of HD in vivo. We generated new mouse models that express mutant Huntington's protein (mhtt), but have partial or total loss of caveolin-1 (Cav1) expression. Fluorescence resonance energy transfer dequenching confirms a direct interaction between mhtt and Cav1. Mhtt-expressing neurons exhibited cholesterol accumulation and suppressed caveolar-related post-Golgi trafficking from endoplasmic reticulum/Golgi to plasma membrane. Loss or reduction of Cav1 expression in a knock-in HD mouse model rescues the cholesterol phenotype in neurons and significantly delays the onset of motor decline and development of neuronal inclusions. We propose that aberrant interaction between Cav1 and mhtt leads to altered cholesterol homeostasis and plays a direct causative role in the onset of HD pathophysiology in vivo.

Original languageEnglish (US)
Article numberddt406
Pages (from-to)129-144
Number of pages16
JournalHuman Molecular Genetics
Volume23
Issue number1
DOIs
StatePublished - Jan 2014

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Caveolin 1
Huntington Disease
Mutant Proteins
Cholesterol
Neurons
Fluorescence Resonance Energy Transfer
Endoplasmic Reticulum
Homeostasis
Cell Membrane
Phenotype

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)
  • Molecular Biology

Cite this

Loss of caveolin-1 expression in knock-in mouse model of huntington's disease suppresses pathophysiology in vivo. / Trushina, Eugenia D; Canaria, Christie A.; Lee, Do Yup; McMurray, Cynthia T.

In: Human Molecular Genetics, Vol. 23, No. 1, ddt406, 01.2014, p. 129-144.

Research output: Contribution to journalArticle

Trushina, Eugenia D ; Canaria, Christie A. ; Lee, Do Yup ; McMurray, Cynthia T. / Loss of caveolin-1 expression in knock-in mouse model of huntington's disease suppresses pathophysiology in vivo. In: Human Molecular Genetics. 2014 ; Vol. 23, No. 1. pp. 129-144.
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