Loss of blast heterogeneity in myelodysplastic syndrome and other chronic myeloid neoplasms

Dragan Jevremovic; Michael M. Timm; Kaaren K. Reichard; William G. Morice; Curtis A. Hanson; David S. Viswanatha; Matthew T. Howard; Phuong L. Nguyen

doi:10.1309/AJCP73QSLLYDEGXK

Loss of blast heterogeneity in myelodysplastic syndrome and other chronic myeloid neoplasms

Dragan Jevremovic, Michael M. Timm, Kaaren K. Reichard, William G. Morice, Curtis A. Hanson, David S. Viswanatha, Matthew T. Howard, Phuong L. Nguyen

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Objectives: Flow cytometry immunophenotyping has been suggested as an adjunctive technique in the evaluation of myeloid malignancies, especially in the myelodysplastic syndromes. However, its use has been limited due to complexity and cost restraints. The goal of this study is to attempt a simpler approach to flow cytometry immunophenotyping in myeloid neoplasms.

Methods: We analyzed bone marrow specimens of 45 selected patients and an additional 99 consecutive random patients using a limited antibody panel.

Results: Normal CD34-positive blasts show a characteristic pattern of CD13/HLA-DR expression, with three readily identifiable subpopulations. In contrast, myeloid neoplasms frequently show loss of this heterogeneity.

Conclusions: Analysis of a limited antibody panel with a focus on CD13/HLA-DR expression provides relatively high specificity and sensitivity for the detection of myeloid neoplasms.

Original language	English (US)
Pages (from-to)	292-298
Number of pages	7
Journal	American journal of clinical pathology
Volume	142
Issue number	3
DOIs	https://doi.org/10.1309/AJCP73QSLLYDEGXK
State	Published - Sep 1 2014

Keywords

Blasts
Flow cytometry
MDS

ASJC Scopus subject areas

Pathology and Forensic Medicine

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10.1309/AJCP73QSLLYDEGXK

Cite this

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title = "Loss of blast heterogeneity in myelodysplastic syndrome and other chronic myeloid neoplasms",

abstract = "Objectives: Flow cytometry immunophenotyping has been suggested as an adjunctive technique in the evaluation of myeloid malignancies, especially in the myelodysplastic syndromes. However, its use has been limited due to complexity and cost restraints. The goal of this study is to attempt a simpler approach to flow cytometry immunophenotyping in myeloid neoplasms.Methods: We analyzed bone marrow specimens of 45 selected patients and an additional 99 consecutive random patients using a limited antibody panel.Results: Normal CD34-positive blasts show a characteristic pattern of CD13/HLA-DR expression, with three readily identifiable subpopulations. In contrast, myeloid neoplasms frequently show loss of this heterogeneity.Conclusions: Analysis of a limited antibody panel with a focus on CD13/HLA-DR expression provides relatively high specificity and sensitivity for the detection of myeloid neoplasms.",

keywords = "Blasts, Flow cytometry, MDS",

author = "Dragan Jevremovic and Timm, {Michael M.} and Reichard, {Kaaren K.} and Morice, {William G.} and Hanson, {Curtis A.} and Viswanatha, {David S.} and Howard, {Matthew T.} and Nguyen, {Phuong L.}",

note = "Publisher Copyright: {\textcopyright} American Society for Clinical Pathology.",

year = "2014",

month = sep,

day = "1",

doi = "10.1309/AJCP73QSLLYDEGXK",

language = "English (US)",

volume = "142",

pages = "292--298",

journal = "American Journal of Clinical Pathology",

issn = "0002-9173",

publisher = "American Society of Clinical Pathologists",

number = "3",

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TY - JOUR

T1 - Loss of blast heterogeneity in myelodysplastic syndrome and other chronic myeloid neoplasms

AU - Jevremovic, Dragan

AU - Timm, Michael M.

AU - Reichard, Kaaren K.

AU - Morice, William G.

AU - Hanson, Curtis A.

AU - Viswanatha, David S.

AU - Howard, Matthew T.

AU - Nguyen, Phuong L.

PY - 2014/9/1

Y1 - 2014/9/1

N2 - Objectives: Flow cytometry immunophenotyping has been suggested as an adjunctive technique in the evaluation of myeloid malignancies, especially in the myelodysplastic syndromes. However, its use has been limited due to complexity and cost restraints. The goal of this study is to attempt a simpler approach to flow cytometry immunophenotyping in myeloid neoplasms.Methods: We analyzed bone marrow specimens of 45 selected patients and an additional 99 consecutive random patients using a limited antibody panel.Results: Normal CD34-positive blasts show a characteristic pattern of CD13/HLA-DR expression, with three readily identifiable subpopulations. In contrast, myeloid neoplasms frequently show loss of this heterogeneity.Conclusions: Analysis of a limited antibody panel with a focus on CD13/HLA-DR expression provides relatively high specificity and sensitivity for the detection of myeloid neoplasms.

AB - Objectives: Flow cytometry immunophenotyping has been suggested as an adjunctive technique in the evaluation of myeloid malignancies, especially in the myelodysplastic syndromes. However, its use has been limited due to complexity and cost restraints. The goal of this study is to attempt a simpler approach to flow cytometry immunophenotyping in myeloid neoplasms.Methods: We analyzed bone marrow specimens of 45 selected patients and an additional 99 consecutive random patients using a limited antibody panel.Results: Normal CD34-positive blasts show a characteristic pattern of CD13/HLA-DR expression, with three readily identifiable subpopulations. In contrast, myeloid neoplasms frequently show loss of this heterogeneity.Conclusions: Analysis of a limited antibody panel with a focus on CD13/HLA-DR expression provides relatively high specificity and sensitivity for the detection of myeloid neoplasms.

KW - Blasts

KW - Flow cytometry

KW - MDS

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JO - American Journal of Clinical Pathology

JF - American Journal of Clinical Pathology

IS - 3

ER -

Loss of blast heterogeneity in myelodysplastic syndrome and other chronic myeloid neoplasms

Abstract

Keywords

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