@article{3dc3e8b8ccdb4edc8f0997f00ebf7749,
title = "Loss of a consensus heparin binding site by alternative splicing of latent transforming growth factor-β binding protein-1",
abstract = "Latent transforming growth factor-β binding protein-1 (LTBP-1), plays an important role in controlling localisation and activation of transforming growth factor-β (TGF-β). We show that alternative splicing generates a form of mRNA,which lacks bases 1277-1435 (termed LTBP-1Δ53). The 53 amino acids encoded by these bases include the eighth cysteine of the first cysteine repeat and a consensus heparin binding sequence. Sequencing of genomic clones showed that alternative splicing resulted from the use of an intra-exonic 3' splice acceptor site. The loss of the heparin binding site implies that LTBP-1Δ53 will bind to the extracellular matrix less efficiently than LTBP-1.",
keywords = "Alternative splicing, Cysteine rich repeat, Heparin binding, Latent transforming growth factor-β binding protein",
author = "Rahmi {\"O}kl{\"u} and Metcalfe, {James C.} and Hesketh, {T. Robin} and Kemp, {Paul R.}",
note = "Funding Information: We are grateful to Mike O'Hare, Department of Surgery, University College/Middlesex Medical School, London for cultured human breast cells and to John Lester and Torsten Schwecke of the Department of Biochemistry, University of Cambridge for assistance with sequencing. The chromosome specific gene library (LL02NC02) used in this work was constructed at the Human Genome Center, Biology and Biotechnology Research Program, L-452, Lawrence Livermore National Laboratory, Livermore, CA 94550, under the auspices of the National Laboratory Gene Library Project sponsored by the US Department of Energy and was distributed by the UK HGMP resource centre, Hinxton Hall, Cambridge, UK. Funding Information: This work was supported by a British Heart Foundation program grant to J.C.M. and Dr. David Grainger. P.R.K. is a British Heart Foundation Basic Sciences Lecturer and R.{\"O}. is an Ali Y. Ko{\c c} scholar. ",
year = "1998",
month = mar,
day = "27",
doi = "10.1016/S0014-5793(98)00257-9",
language = "English (US)",
volume = "425",
pages = "281--285",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "2",
}