Long-term depression requires postsynaptic AMPA GluR2 receptor in adult mouse cingulate cortex

Hiroki Toyoda, LongJun Wu, Ming Gao Zhao, Hui Xu, Zhengping Jia, Min Zhuo

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Synaptic long-term depression (LTD) is thought to be important for various brain functions such as learning, memory, and development. Although anterior cingulated cortex (ACC) has been demonstrated to contribute to learning and memory, no studies has been reported about the synaptic mechanisms for cingulate LTD. Here, we used integrative genetic, pharmacological and electrophysiological approaches to demonstrate that AMPA GluR2, but not GluR3, subunit is critical for cingulate LTD. We found that LTD was absent in adult cingulate slices of GluR2 knockout mice. Furthermore, postsynaptic injections of peptides that inhibit AMPA GluR2-PDZ interactions blocked the induction of LTD. To determine if the requirement for AMPA receptor-PDZ interaction is time-dependent, we injected the same inhibiting peptide into the postsynaptic cells 5 min after the induction of LTD. We found that LTD was not affected by the peptide, providing the first evidence that postsynaptic AMPA GluR2-mediated depression occurs rapidly (within t = 5 min). Genetic deletion of GluR3 did not affect cingulate LTD. Our results provide the first study of cingulate LTD mechanism using whole-cell patch-clamp recording in adult cortical slices and demonstrate that postsynaptic AMPA GluR2 subunit is crucial for synaptic depression in the ACC of adult mice.

Original languageEnglish (US)
Pages (from-to)336-343
Number of pages8
JournalJournal of Cellular Physiology
Volume211
Issue number2
DOIs
StatePublished - May 1 2007
Externally publishedYes

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alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
AMPA Receptors
Gyrus Cinguli
Depression
Peptides
Data storage equipment
Clamping devices
Brain
Long-Term Synaptic Depression
Learning
AMPA 2 glutamate receptor ionotropic
Knockout Mice
Pharmacology
Injections

ASJC Scopus subject areas

  • Medicine(all)
  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Long-term depression requires postsynaptic AMPA GluR2 receptor in adult mouse cingulate cortex. / Toyoda, Hiroki; Wu, LongJun; Zhao, Ming Gao; Xu, Hui; Jia, Zhengping; Zhuo, Min.

In: Journal of Cellular Physiology, Vol. 211, No. 2, 01.05.2007, p. 336-343.

Research output: Contribution to journalArticle

Toyoda, Hiroki ; Wu, LongJun ; Zhao, Ming Gao ; Xu, Hui ; Jia, Zhengping ; Zhuo, Min. / Long-term depression requires postsynaptic AMPA GluR2 receptor in adult mouse cingulate cortex. In: Journal of Cellular Physiology. 2007 ; Vol. 211, No. 2. pp. 336-343.
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