Long-term combined beneficial effects of physical training and metabolic treatment on atherosclerosis in hypercholesterolemic mice

Claudio Napoli, Sharon Williams-Ignarro, Filomena De Nigris, Lilach O Lerman, Loredana Rossi, Carmen Guarino, Gelsomina Mansueto, Francesco Di Tuoro, Orlando Pignalosa, Gaetano De Rosa, Vincenzo Sica, Louis J. Ignarro

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

The pathogenic mechanisms by which physical exercise influences atherosclerotic lesion formation remain poorly understood. Because vigorous physical training increases oxidative stress, this study tested the hypothesis that graduated and moderate physical exercise together with metabolic intervention (L-arginine and antioxidants) may contribute to increased vascular protection. Exercise training in mice was induced by graduated swimming. In hypercholesterolemic male mice on an atherogenic high-cholesterol diet, graduated and moderate exercise lowered plasma cholesterol and decreased atherosclerotic lesions compared with sedentary control mice. Antioxidants (1. 0% vitamin E added to the chow and 0.05% vitamin C added to the drinking water) and L-arginine (6% in drinking water) supplementation to exercising hypercholesterolemic mice further and synergistically reduced atherosclerosis compared with untreated exercised mice. Arterial oxidation-specific epitopes and systemic oxidative stress were reduced by metabolic intervention. Graduated chronic exercise elicited an increase in production of nitric oxide through increased endothelial nitric oxide synthase expression and ameliorated scavenger activities. Thus, metabolic intervention with L-arginine and antioxidants together with graduated and moderate exercise training reduce atherosclerotic lesion formation.

Original languageEnglish (US)
Pages (from-to)8797-8802
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume101
Issue number23
DOIs
StatePublished - Jun 8 2004

Fingerprint

Atherosclerosis
Exercise
Arginine
Antioxidants
Drinking Water
Oxidative Stress
Cholesterol
Nitric Oxide Synthase Type III
Vitamin E
Ascorbic Acid
Blood Vessels
Epitopes
Nitric Oxide
Diet

Keywords

  • Catalase
  • Low-density lipoprotein
  • Nitric oxide synthase
  • Oxidative stress
  • Vitamin E

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Long-term combined beneficial effects of physical training and metabolic treatment on atherosclerosis in hypercholesterolemic mice. / Napoli, Claudio; Williams-Ignarro, Sharon; De Nigris, Filomena; Lerman, Lilach O; Rossi, Loredana; Guarino, Carmen; Mansueto, Gelsomina; Di Tuoro, Francesco; Pignalosa, Orlando; De Rosa, Gaetano; Sica, Vincenzo; Ignarro, Louis J.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, No. 23, 08.06.2004, p. 8797-8802.

Research output: Contribution to journalArticle

Napoli, C, Williams-Ignarro, S, De Nigris, F, Lerman, LO, Rossi, L, Guarino, C, Mansueto, G, Di Tuoro, F, Pignalosa, O, De Rosa, G, Sica, V & Ignarro, LJ 2004, 'Long-term combined beneficial effects of physical training and metabolic treatment on atherosclerosis in hypercholesterolemic mice', Proceedings of the National Academy of Sciences of the United States of America, vol. 101, no. 23, pp. 8797-8802. https://doi.org/10.1073/pnas.0402734101
Napoli, Claudio ; Williams-Ignarro, Sharon ; De Nigris, Filomena ; Lerman, Lilach O ; Rossi, Loredana ; Guarino, Carmen ; Mansueto, Gelsomina ; Di Tuoro, Francesco ; Pignalosa, Orlando ; De Rosa, Gaetano ; Sica, Vincenzo ; Ignarro, Louis J. / Long-term combined beneficial effects of physical training and metabolic treatment on atherosclerosis in hypercholesterolemic mice. In: Proceedings of the National Academy of Sciences of the United States of America. 2004 ; Vol. 101, No. 23. pp. 8797-8802.
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