Long-term bovine hematopoietic engraftment with clone-derived stem cells

Robert Lanza, J. H. Shieh, Peter J. Wettstein, Raymond W. Sweeney, Kaida Wu, Anat Weisz, Nancy Borson, Boyd Henderson, Michael D. West, Malcolm A.S. Moore

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Therapeutic cloning by somatic cell nuclear transfer offers potential for treatment of a wide range of degenerative disease. Nuclear transplantation with Meor-marked somatic nuclei from 10-13-year-old cows was used to generate cloned bovine fetuses. Clone fetal liver (FL) hematopoietic stem cells (HSC) were transplanted into two busulfan-treated and one untreated nuclear donor cows. Hematopoiesis was monitored over 13-16 months by in vitro progenitor and HSC assays. Chimerism was demonstrated by PCR in blood, marrow, lymph nodes, and endothelium, peaking at levels of 9-17% in blood granulocytes but at lower levels in lymphocyte subsets (0.1-0.01%). Circulating progenitors showed high levels of chimerism (up to 60% neor+) with persisting fetal features. At sacrifice, the animal that had no pre-transplant myelosupression showed persisting donor cells in blood and lymph nodes, and in marrow 0.25% of progenitor cells and a detectable fraction of stem cells were neor+. The fetal HSC showed a 10-fold competition advantage over adult HSC. Cloning generated histocompatible HSC capable of long-term multilineage engraftment in a large animal model.

Original languageEnglish (US)
Pages (from-to)95-106
Number of pages12
JournalCloning and Stem Cells
Volume7
Issue number2
DOIs
StatePublished - 2005

ASJC Scopus subject areas

  • Biotechnology
  • Developmental Biology

Fingerprint Dive into the research topics of 'Long-term bovine hematopoietic engraftment with clone-derived stem cells'. Together they form a unique fingerprint.

Cite this