Localization of three novel hybrid breakpoints and refinement of 18 marker assignments in the human 3cen-p21.1 region

Nai dy Wang, Joseph R. Testa, David I. Smith

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Using the human/hamster cell line UCTP2A-3, we have generated and isolated three hybrids, each containing a novel human chromosome 3p break. All chromosome 3 materials distal to the breaks were lost. Two of the breakpoints were produced using aphidicolin induction; the third breakpoint occurred spontaneously. The aphidicolin-induced breaks were localized to 3p21.1 in hybrid AR1 and to p14.1 in hybrid AR2. The spontaneous break was localized to 3p11 in hybrid 2A-3-1. These hybrids were used to sublocalize 18 chromosome 3 probes further to five regions within 3cen-p21.1. The new hybrids and sublocalized markers will be useful in the study and characterization of the 3p11, 3p14.1, and 3p21.1 segments of chromosome 3.

Original languageEnglish (US)
Pages (from-to)891-896
Number of pages6
JournalGenomics
Volume14
Issue number4
DOIs
StatePublished - Dec 1992

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ASJC Scopus subject areas

  • Genetics

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