Localization of PS6K to chromosomal region 17q23 and determination of its amplification in breast cancer

Fergus J. Couch, Xiao Yang Wang, Guo Jun Wu, Junqi Qian, Robert B. Jenkins, C. David James

Research output: Contribution to journalArticle

92 Scopus citations

Abstract

The application of comparative genomic hybridization to the analysis of genetic abnormalities in breast carcinoma has consistently revealed that chromosome region 17q22-24 is a frequent site of gene amplification in this type of cancer. As part of an examination of expressed sequence tags for novel amplified genes in this region, we identified PS6K amplifications in both breast tumor tissues and cell lines. PS6K was localized to 17q23 and encodes a serine-threonine kinase whose activation is thought to regulate a wide array of cellular processes involved in the mitogenic response including protein synthesis, translation of specific mRNA species, and cell cycle progression from G1 to S phase. Northern and Western analyses revealed that amplification of this gene was accompanied by corresponding increases in mRNA and protein expression, respectively. These data represent the first determination of a gene amplification within 17q22-24 in breast cancer and suggest an oncogenic activity for PS6K.

Original languageEnglish (US)
Pages (from-to)1408-1411
Number of pages4
JournalCancer research
Volume59
Issue number7
StatePublished - Apr 1 1999

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Localization of PS6K to chromosomal region 17q23 and determination of its amplification in breast cancer'. Together they form a unique fingerprint.

  • Cite this