Localization of latent transforming growth factor-β binding protein-1 in human coronary atherosclerotic plaques

Rahmi Oklu, Robin Hesketh, Stephan Wicky, James C. Metcalfe

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: Transforming growth factor-β (TGFβ) and its receptors have been detected by immunohisto-chemistry in the normal vessel wall and in atherosclerotic lesions of human coronary arteries. However, TGF/S is normally secreted as an inactive complex associated with a latent TGFβ-binding protein (LTBP). Therefore, detection of TGFβ antigen only in the arterial wall does not imply the activated form of the growth factor. Methods and Results: In situ hybridization and immunohistochemistry demonstrated LTBP1 mRNA and protein expression throughout the media and intima of early coronary artery lesions, with the highest levels of protein at the luminal surface. In advanced lesions, LTBP1 mRNA and protein were detected mainly in regions of high cell density, such as the fibrous cap. Conclusions: Assays of the TGFβ signalling pathway will be required to determine the activity associated with TGFβ antigen in the vessel wall.

Original languageEnglish (US)
Pages (from-to)196-200
Number of pages5
JournalCirculation Journal
Volume75
Issue number1
DOIs
StatePublished - 2011
Externally publishedYes

Fingerprint

Transforming Growth Factors
Atherosclerotic Plaques
Carrier Proteins
Coronary Vessels
Antigens
Messenger RNA
Proteins
Growth Factor Receptors
In Situ Hybridization
Intercellular Signaling Peptides and Proteins
Cell Count
Immunohistochemistry

Keywords

  • Atherosclerosis
  • Coronary heart disease
  • Genes
  • Immunohistochemistry
  • Molecular biology

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Localization of latent transforming growth factor-β binding protein-1 in human coronary atherosclerotic plaques. / Oklu, Rahmi; Hesketh, Robin; Wicky, Stephan; Metcalfe, James C.

In: Circulation Journal, Vol. 75, No. 1, 2011, p. 196-200.

Research output: Contribution to journalArticle

Oklu, Rahmi ; Hesketh, Robin ; Wicky, Stephan ; Metcalfe, James C. / Localization of latent transforming growth factor-β binding protein-1 in human coronary atherosclerotic plaques. In: Circulation Journal. 2011 ; Vol. 75, No. 1. pp. 196-200.
@article{975e30d0765d4a5c80828ecc3c560c62,
title = "Localization of latent transforming growth factor-β binding protein-1 in human coronary atherosclerotic plaques",
abstract = "Background: Transforming growth factor-β (TGFβ) and its receptors have been detected by immunohisto-chemistry in the normal vessel wall and in atherosclerotic lesions of human coronary arteries. However, TGF/S is normally secreted as an inactive complex associated with a latent TGFβ-binding protein (LTBP). Therefore, detection of TGFβ antigen only in the arterial wall does not imply the activated form of the growth factor. Methods and Results: In situ hybridization and immunohistochemistry demonstrated LTBP1 mRNA and protein expression throughout the media and intima of early coronary artery lesions, with the highest levels of protein at the luminal surface. In advanced lesions, LTBP1 mRNA and protein were detected mainly in regions of high cell density, such as the fibrous cap. Conclusions: Assays of the TGFβ signalling pathway will be required to determine the activity associated with TGFβ antigen in the vessel wall.",
keywords = "Atherosclerosis, Coronary heart disease, Genes, Immunohistochemistry, Molecular biology",
author = "Rahmi Oklu and Robin Hesketh and Stephan Wicky and Metcalfe, {James C.}",
year = "2011",
doi = "10.1253/circj.CJ-10-0334",
language = "English (US)",
volume = "75",
pages = "196--200",
journal = "Circulation Journal",
issn = "1346-9843",
publisher = "Japanese Circulation Society",
number = "1",

}

TY - JOUR

T1 - Localization of latent transforming growth factor-β binding protein-1 in human coronary atherosclerotic plaques

AU - Oklu, Rahmi

AU - Hesketh, Robin

AU - Wicky, Stephan

AU - Metcalfe, James C.

PY - 2011

Y1 - 2011

N2 - Background: Transforming growth factor-β (TGFβ) and its receptors have been detected by immunohisto-chemistry in the normal vessel wall and in atherosclerotic lesions of human coronary arteries. However, TGF/S is normally secreted as an inactive complex associated with a latent TGFβ-binding protein (LTBP). Therefore, detection of TGFβ antigen only in the arterial wall does not imply the activated form of the growth factor. Methods and Results: In situ hybridization and immunohistochemistry demonstrated LTBP1 mRNA and protein expression throughout the media and intima of early coronary artery lesions, with the highest levels of protein at the luminal surface. In advanced lesions, LTBP1 mRNA and protein were detected mainly in regions of high cell density, such as the fibrous cap. Conclusions: Assays of the TGFβ signalling pathway will be required to determine the activity associated with TGFβ antigen in the vessel wall.

AB - Background: Transforming growth factor-β (TGFβ) and its receptors have been detected by immunohisto-chemistry in the normal vessel wall and in atherosclerotic lesions of human coronary arteries. However, TGF/S is normally secreted as an inactive complex associated with a latent TGFβ-binding protein (LTBP). Therefore, detection of TGFβ antigen only in the arterial wall does not imply the activated form of the growth factor. Methods and Results: In situ hybridization and immunohistochemistry demonstrated LTBP1 mRNA and protein expression throughout the media and intima of early coronary artery lesions, with the highest levels of protein at the luminal surface. In advanced lesions, LTBP1 mRNA and protein were detected mainly in regions of high cell density, such as the fibrous cap. Conclusions: Assays of the TGFβ signalling pathway will be required to determine the activity associated with TGFβ antigen in the vessel wall.

KW - Atherosclerosis

KW - Coronary heart disease

KW - Genes

KW - Immunohistochemistry

KW - Molecular biology

UR - http://www.scopus.com/inward/record.url?scp=78651225686&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78651225686&partnerID=8YFLogxK

U2 - 10.1253/circj.CJ-10-0334

DO - 10.1253/circj.CJ-10-0334

M3 - Article

C2 - 21071877

AN - SCOPUS:78651225686

VL - 75

SP - 196

EP - 200

JO - Circulation Journal

JF - Circulation Journal

SN - 1346-9843

IS - 1

ER -