Localization of human eosinophil granule major basic protein, eosinophil cationic protein, and eosinophil-derived neurotoxin by immunoelectron microscopy

M. S. Peters, M. Rodriguez, G. J. Gleich

Research output: Contribution to journalArticle

125 Citations (Scopus)

Abstract

By utilizing the colloidal gold particle technique, we localized eosinophil granule major basic protein, eosinophil cationic protein (ECP), and eosinophil-derived neurotoxin (EDN) in human nasal polyp sections by immunoelectron microscopy. Sections stained with affinity chromatography purified rabbit anti-human major basic protein, and subsequently with gold colloidal particle-goat anti-rabbit IgG, showed gold particles predominantly within granule cores, and not within other eosinophil organelles, plasma cells, mast cells, lymphocytes, or neutrophils. Sections stained with anti-ECP or anti-EDN showed gold particles concentrated over the granule matrix with fewer particles centrally. Control sections treated with preimmunization sera showed no staining of cells or organelles. These results verify the localization of major basic protein to the crystalloid core of the human eosinophil granule and show that ECP and EDN reside in the granule matrix. This technique provides a means of accurately locating the sites of major basic protein, ECP, and EDN deposition and thus of identifying eosinophil degranulation patterns in human disease.

Original languageEnglish (US)
Pages (from-to)656-662
Number of pages7
JournalLaboratory Investigation
Volume54
Issue number6
StatePublished - 1986

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Eosinophil-Derived Neurotoxin
Eosinophil Cationic Protein
Immunoelectron Microscopy
Eosinophils
Gold Colloid
Gold
Organelles
Eosinophil Major Basic Protein
Rabbits
Nasal Polyps
Proteins
Plasma Cells
Affinity Chromatography
Goats
Mast Cells
Neutrophils
Lymphocytes
Staining and Labeling
human PRG2 protein
Serum

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Localization of human eosinophil granule major basic protein, eosinophil cationic protein, and eosinophil-derived neurotoxin by immunoelectron microscopy. / Peters, M. S.; Rodriguez, M.; Gleich, G. J.

In: Laboratory Investigation, Vol. 54, No. 6, 1986, p. 656-662.

Research output: Contribution to journalArticle

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AB - By utilizing the colloidal gold particle technique, we localized eosinophil granule major basic protein, eosinophil cationic protein (ECP), and eosinophil-derived neurotoxin (EDN) in human nasal polyp sections by immunoelectron microscopy. Sections stained with affinity chromatography purified rabbit anti-human major basic protein, and subsequently with gold colloidal particle-goat anti-rabbit IgG, showed gold particles predominantly within granule cores, and not within other eosinophil organelles, plasma cells, mast cells, lymphocytes, or neutrophils. Sections stained with anti-ECP or anti-EDN showed gold particles concentrated over the granule matrix with fewer particles centrally. Control sections treated with preimmunization sera showed no staining of cells or organelles. These results verify the localization of major basic protein to the crystalloid core of the human eosinophil granule and show that ECP and EDN reside in the granule matrix. This technique provides a means of accurately locating the sites of major basic protein, ECP, and EDN deposition and thus of identifying eosinophil degranulation patterns in human disease.

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