Localization of common deletion regions on 1p and 19q in human gliomas and their association with histological subtype

Justin S. Smith, Benjamin Alderete, Yuriko Minn, Thomas J. Borell, Arie Perry, Gayatry Mohapatra, Sandra M. Hosek, David Kimmel, Judith O'Fallon, Allan Yates, Burt G. Feuerstein, Peter C. Burger, Bernd W. Scheithauer, Robert Brian Jenkins

Research output: Contribution to journalArticle

311 Citations (Scopus)

Abstract

Allelic alterations of chromosomes 1 and 19 are frequent events in human diffuse gliomas and have recently proven to be strong predictors of chemotherapeutic response and prolonged survival in oligodendrogliomas. Using 115 human diffuse gliomas, we localized regions of common allelic loss on chromosomes 1 and 19 and assessed the association of these deletion intervals with glioma histological subtypes. Further, we evaluated the capacity of multiple modalities to detect these alterations, including loss of heterozygosity (LOH), fluorescence in situ hybridization (FISH), and comparative genomic hybridization (CGH). The correlation coefficients for detection of 1p and 19q alterations, respectively, between modalities were: 0.98 and 0.87 for LOH and FISH, 0.79 and 0.60 for LOH and CGH, and 0.79 and 0.53 for FISH and CGH. Minimal deletion regions were defined on 19q13.3 (D19S412-D19S596) and 1p (D1S468-D1S1612). Loss of the 1p36 region was found in 18% of astrocytomas (10/55) and in 73% (24/33) of oligodendrogliomas (P < 0.0001), and loss of the 19q13.3 region was found in 38% (21/55) of astrocytomas and 73% (24/33) of oligodendrogliomas (P = 0.0017). Loss of both regions was found in 11% (6/55) of astrocytomas and in 64% (21/33) of oligodendrogliomas (P < 0.0001). All gliomas with LOH on either 1p or 19q demonstrated loss of the corresponding FISH probe, 1p36 or 19q13.3, suggesting not only locations of putative tumor suppressor genes, but also a simple assay for assessment of 1p and 19q alterations as diagnostic and prognostic markers.

Original languageEnglish (US)
Pages (from-to)4144-4152
Number of pages9
JournalOncogene
Volume18
Issue number28
DOIs
StatePublished - Jul 15 1999

Fingerprint

Loss of Heterozygosity
Oligodendroglioma
Glioma
Fluorescence In Situ Hybridization
Comparative Genomic Hybridization
Astrocytoma
Chromosomes, Human, Pair 19
Chromosomes, Human, Pair 1
Tumor Suppressor Genes
Survival

Keywords

  • Chromosome 1
  • Chromosome 19
  • Comparative genomic hybridization
  • Diffuse glioma
  • Fluorescence in situ hybridization
  • Loss of heterozygosity
  • Tumor suppressor gene

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Localization of common deletion regions on 1p and 19q in human gliomas and their association with histological subtype. / Smith, Justin S.; Alderete, Benjamin; Minn, Yuriko; Borell, Thomas J.; Perry, Arie; Mohapatra, Gayatry; Hosek, Sandra M.; Kimmel, David; O'Fallon, Judith; Yates, Allan; Feuerstein, Burt G.; Burger, Peter C.; Scheithauer, Bernd W.; Jenkins, Robert Brian.

In: Oncogene, Vol. 18, No. 28, 15.07.1999, p. 4144-4152.

Research output: Contribution to journalArticle

Smith, JS, Alderete, B, Minn, Y, Borell, TJ, Perry, A, Mohapatra, G, Hosek, SM, Kimmel, D, O'Fallon, J, Yates, A, Feuerstein, BG, Burger, PC, Scheithauer, BW & Jenkins, RB 1999, 'Localization of common deletion regions on 1p and 19q in human gliomas and their association with histological subtype', Oncogene, vol. 18, no. 28, pp. 4144-4152. https://doi.org/10.1038/sj.onc.1202759
Smith, Justin S. ; Alderete, Benjamin ; Minn, Yuriko ; Borell, Thomas J. ; Perry, Arie ; Mohapatra, Gayatry ; Hosek, Sandra M. ; Kimmel, David ; O'Fallon, Judith ; Yates, Allan ; Feuerstein, Burt G. ; Burger, Peter C. ; Scheithauer, Bernd W. ; Jenkins, Robert Brian. / Localization of common deletion regions on 1p and 19q in human gliomas and their association with histological subtype. In: Oncogene. 1999 ; Vol. 18, No. 28. pp. 4144-4152.
@article{5f2b9a55438b4c61ae6962666b8e9963,
title = "Localization of common deletion regions on 1p and 19q in human gliomas and their association with histological subtype",
abstract = "Allelic alterations of chromosomes 1 and 19 are frequent events in human diffuse gliomas and have recently proven to be strong predictors of chemotherapeutic response and prolonged survival in oligodendrogliomas. Using 115 human diffuse gliomas, we localized regions of common allelic loss on chromosomes 1 and 19 and assessed the association of these deletion intervals with glioma histological subtypes. Further, we evaluated the capacity of multiple modalities to detect these alterations, including loss of heterozygosity (LOH), fluorescence in situ hybridization (FISH), and comparative genomic hybridization (CGH). The correlation coefficients for detection of 1p and 19q alterations, respectively, between modalities were: 0.98 and 0.87 for LOH and FISH, 0.79 and 0.60 for LOH and CGH, and 0.79 and 0.53 for FISH and CGH. Minimal deletion regions were defined on 19q13.3 (D19S412-D19S596) and 1p (D1S468-D1S1612). Loss of the 1p36 region was found in 18{\%} of astrocytomas (10/55) and in 73{\%} (24/33) of oligodendrogliomas (P < 0.0001), and loss of the 19q13.3 region was found in 38{\%} (21/55) of astrocytomas and 73{\%} (24/33) of oligodendrogliomas (P = 0.0017). Loss of both regions was found in 11{\%} (6/55) of astrocytomas and in 64{\%} (21/33) of oligodendrogliomas (P < 0.0001). All gliomas with LOH on either 1p or 19q demonstrated loss of the corresponding FISH probe, 1p36 or 19q13.3, suggesting not only locations of putative tumor suppressor genes, but also a simple assay for assessment of 1p and 19q alterations as diagnostic and prognostic markers.",
keywords = "Chromosome 1, Chromosome 19, Comparative genomic hybridization, Diffuse glioma, Fluorescence in situ hybridization, Loss of heterozygosity, Tumor suppressor gene",
author = "Smith, {Justin S.} and Benjamin Alderete and Yuriko Minn and Borell, {Thomas J.} and Arie Perry and Gayatry Mohapatra and Hosek, {Sandra M.} and David Kimmel and Judith O'Fallon and Allan Yates and Feuerstein, {Burt G.} and Burger, {Peter C.} and Scheithauer, {Bernd W.} and Jenkins, {Robert Brian}",
year = "1999",
month = "7",
day = "15",
doi = "10.1038/sj.onc.1202759",
language = "English (US)",
volume = "18",
pages = "4144--4152",
journal = "Oncogene",
issn = "0950-9232",
publisher = "Nature Publishing Group",
number = "28",

}

TY - JOUR

T1 - Localization of common deletion regions on 1p and 19q in human gliomas and their association with histological subtype

AU - Smith, Justin S.

AU - Alderete, Benjamin

AU - Minn, Yuriko

AU - Borell, Thomas J.

AU - Perry, Arie

AU - Mohapatra, Gayatry

AU - Hosek, Sandra M.

AU - Kimmel, David

AU - O'Fallon, Judith

AU - Yates, Allan

AU - Feuerstein, Burt G.

AU - Burger, Peter C.

AU - Scheithauer, Bernd W.

AU - Jenkins, Robert Brian

PY - 1999/7/15

Y1 - 1999/7/15

N2 - Allelic alterations of chromosomes 1 and 19 are frequent events in human diffuse gliomas and have recently proven to be strong predictors of chemotherapeutic response and prolonged survival in oligodendrogliomas. Using 115 human diffuse gliomas, we localized regions of common allelic loss on chromosomes 1 and 19 and assessed the association of these deletion intervals with glioma histological subtypes. Further, we evaluated the capacity of multiple modalities to detect these alterations, including loss of heterozygosity (LOH), fluorescence in situ hybridization (FISH), and comparative genomic hybridization (CGH). The correlation coefficients for detection of 1p and 19q alterations, respectively, between modalities were: 0.98 and 0.87 for LOH and FISH, 0.79 and 0.60 for LOH and CGH, and 0.79 and 0.53 for FISH and CGH. Minimal deletion regions were defined on 19q13.3 (D19S412-D19S596) and 1p (D1S468-D1S1612). Loss of the 1p36 region was found in 18% of astrocytomas (10/55) and in 73% (24/33) of oligodendrogliomas (P < 0.0001), and loss of the 19q13.3 region was found in 38% (21/55) of astrocytomas and 73% (24/33) of oligodendrogliomas (P = 0.0017). Loss of both regions was found in 11% (6/55) of astrocytomas and in 64% (21/33) of oligodendrogliomas (P < 0.0001). All gliomas with LOH on either 1p or 19q demonstrated loss of the corresponding FISH probe, 1p36 or 19q13.3, suggesting not only locations of putative tumor suppressor genes, but also a simple assay for assessment of 1p and 19q alterations as diagnostic and prognostic markers.

AB - Allelic alterations of chromosomes 1 and 19 are frequent events in human diffuse gliomas and have recently proven to be strong predictors of chemotherapeutic response and prolonged survival in oligodendrogliomas. Using 115 human diffuse gliomas, we localized regions of common allelic loss on chromosomes 1 and 19 and assessed the association of these deletion intervals with glioma histological subtypes. Further, we evaluated the capacity of multiple modalities to detect these alterations, including loss of heterozygosity (LOH), fluorescence in situ hybridization (FISH), and comparative genomic hybridization (CGH). The correlation coefficients for detection of 1p and 19q alterations, respectively, between modalities were: 0.98 and 0.87 for LOH and FISH, 0.79 and 0.60 for LOH and CGH, and 0.79 and 0.53 for FISH and CGH. Minimal deletion regions were defined on 19q13.3 (D19S412-D19S596) and 1p (D1S468-D1S1612). Loss of the 1p36 region was found in 18% of astrocytomas (10/55) and in 73% (24/33) of oligodendrogliomas (P < 0.0001), and loss of the 19q13.3 region was found in 38% (21/55) of astrocytomas and 73% (24/33) of oligodendrogliomas (P = 0.0017). Loss of both regions was found in 11% (6/55) of astrocytomas and in 64% (21/33) of oligodendrogliomas (P < 0.0001). All gliomas with LOH on either 1p or 19q demonstrated loss of the corresponding FISH probe, 1p36 or 19q13.3, suggesting not only locations of putative tumor suppressor genes, but also a simple assay for assessment of 1p and 19q alterations as diagnostic and prognostic markers.

KW - Chromosome 1

KW - Chromosome 19

KW - Comparative genomic hybridization

KW - Diffuse glioma

KW - Fluorescence in situ hybridization

KW - Loss of heterozygosity

KW - Tumor suppressor gene

UR - http://www.scopus.com/inward/record.url?scp=0033566061&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033566061&partnerID=8YFLogxK

U2 - 10.1038/sj.onc.1202759

DO - 10.1038/sj.onc.1202759

M3 - Article

VL - 18

SP - 4144

EP - 4152

JO - Oncogene

JF - Oncogene

SN - 0950-9232

IS - 28

ER -