Localization of 616 human chromosome 3-specific cosmids using a somatic cell hybrid deletion mapping panel

David I. Smith, Wanguo Liu, David Ginzinger, Patricia Green, Scott Smith, Nai dy Wang, Franco Recchia, Kathy Carolyn, Harry Drabkin, William Golembieski

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16 Scopus citations

Abstract

A total of 5700 human chromosome 3-specific cosmid clones was isolated from a series of cosmid libraries constructed from somatic cell hybrids whose only human component was an entire chromosome 3 or a chromosome 3 containing an interstitial deletion removing 50% of long arm sequences. Several unique sequence chromosome 3-specific hybridization probes were isolated from each of 616 of these cosmids. These probes were then used to localize the cosmids by hybridization to a somatic cell hybrid deletion mapping panel capable of resolving chromosome 3 into nine distinct subregions. All 616 of the cosmids were localized to either the long or short arm of chromosome 3 and 63% of the short arm cosmids were more precisely localized. We have identified a total of 87 cosmids that contain fragments that are evolutionarily conserved. Fragments from these cosmids should prove useful in the identification of new chromosome 3-specific genes as well as in comparative mapping studies. The localized cosmids should provide excellent saturation of human chromosome 3 and facilitate the construction of physical and genetic linkage maps to identify various disease loci including Von Hippel Lindau disease and renal and small cell lung carcinoma.

Original languageEnglish (US)
Pages (from-to)179-187
Number of pages9
JournalGenomics
Volume11
Issue number1
DOIs
StatePublished - Sep 1991

ASJC Scopus subject areas

  • Genetics

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    Smith, D. I., Liu, W., Ginzinger, D., Green, P., Smith, S., Wang, N. D., Recchia, F., Carolyn, K., Drabkin, H., & Golembieski, W. (1991). Localization of 616 human chromosome 3-specific cosmids using a somatic cell hybrid deletion mapping panel. Genomics, 11(1), 179-187. https://doi.org/10.1016/0888-7543(91)90116-V