Localization and possible interactions of catecholamine-and NADPH-diaphorase neurons in human medullary autonomic regions

Eduardo E. Benarroch, Inge L. Smithson, Phillip Anson Low

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The human medulla contains catecholamine-and NADPH-diaphorase (NADPH-d) neurons in both the ventrolateral medulla (VLM) and nucleus of the solitary tract (NTS). There is abundant experimental evidence for the critical role of these areas in control of arterial pressure. We sought to determine the pattern of distribution and topographic relationhip between tyrosine hydroxylase (TH)-immunoreactive and NADPH-d-reactive cell groups in normal human VLM and NTS, in view of their potential implications in human autonomic control and involvement in central autonomic disorders. Medullae from three patients with no neurologic disease were obtained at autopsy within 24 h of death. Individual sections, obtained from the rostral and caudal medulla, were stained for TH, NADPH-d or both. We found that: (1) TH-and NADPH-d positive neurons are topographically segregated in the VLM; (2) in the VLM, TH and NADPH-d neurons may coexist within a given area but both markers do not appear to coexist in single neuron; (3) NADPH-d-reactive fibers and processes overlap the distribution of TH neurons within the VLM; and (4) both TH-and NADPH-d-reactive processes appear to innervate intrinsic blood vessels in the VLM and NTS. Thus, there are important topographic relationships between catecholamine-and NO-synthesizing neurons in human VLM and perhaps NTS, including innervation of intrinsic blood vessels. This may have important implications in regulation of autonomic reflexes, sympathetic excitatory drive and intrinsic control of cerebral blood flow in humans.

Original languageEnglish (US)
Pages (from-to)215-220
Number of pages6
JournalBrain Research
Volume684
Issue number2
DOIs
StatePublished - Jul 3 1995

Fingerprint

NADPH Dehydrogenase
Tyrosine 3-Monooxygenase
Catecholamines
Neurons
Blood Vessels
Cerebrovascular Circulation
Solitary Nucleus
Nervous System Diseases
Reflex
Autopsy
Arterial Pressure

Keywords

  • Catecholamine
  • Cerebral blood vessel
  • Human medulla
  • Nitric oxide
  • Ventrolateral medulla

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Localization and possible interactions of catecholamine-and NADPH-diaphorase neurons in human medullary autonomic regions. / Benarroch, Eduardo E.; Smithson, Inge L.; Low, Phillip Anson.

In: Brain Research, Vol. 684, No. 2, 03.07.1995, p. 215-220.

Research output: Contribution to journalArticle

@article{bccf5e203dcd4d3b89bbc66a3d395313,
title = "Localization and possible interactions of catecholamine-and NADPH-diaphorase neurons in human medullary autonomic regions",
abstract = "The human medulla contains catecholamine-and NADPH-diaphorase (NADPH-d) neurons in both the ventrolateral medulla (VLM) and nucleus of the solitary tract (NTS). There is abundant experimental evidence for the critical role of these areas in control of arterial pressure. We sought to determine the pattern of distribution and topographic relationhip between tyrosine hydroxylase (TH)-immunoreactive and NADPH-d-reactive cell groups in normal human VLM and NTS, in view of their potential implications in human autonomic control and involvement in central autonomic disorders. Medullae from three patients with no neurologic disease were obtained at autopsy within 24 h of death. Individual sections, obtained from the rostral and caudal medulla, were stained for TH, NADPH-d or both. We found that: (1) TH-and NADPH-d positive neurons are topographically segregated in the VLM; (2) in the VLM, TH and NADPH-d neurons may coexist within a given area but both markers do not appear to coexist in single neuron; (3) NADPH-d-reactive fibers and processes overlap the distribution of TH neurons within the VLM; and (4) both TH-and NADPH-d-reactive processes appear to innervate intrinsic blood vessels in the VLM and NTS. Thus, there are important topographic relationships between catecholamine-and NO-synthesizing neurons in human VLM and perhaps NTS, including innervation of intrinsic blood vessels. This may have important implications in regulation of autonomic reflexes, sympathetic excitatory drive and intrinsic control of cerebral blood flow in humans.",
keywords = "Catecholamine, Cerebral blood vessel, Human medulla, Nitric oxide, Ventrolateral medulla",
author = "Benarroch, {Eduardo E.} and Smithson, {Inge L.} and Low, {Phillip Anson}",
year = "1995",
month = "7",
day = "3",
doi = "10.1016/0006-8993(95)00400-K",
language = "English (US)",
volume = "684",
pages = "215--220",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Localization and possible interactions of catecholamine-and NADPH-diaphorase neurons in human medullary autonomic regions

AU - Benarroch, Eduardo E.

AU - Smithson, Inge L.

AU - Low, Phillip Anson

PY - 1995/7/3

Y1 - 1995/7/3

N2 - The human medulla contains catecholamine-and NADPH-diaphorase (NADPH-d) neurons in both the ventrolateral medulla (VLM) and nucleus of the solitary tract (NTS). There is abundant experimental evidence for the critical role of these areas in control of arterial pressure. We sought to determine the pattern of distribution and topographic relationhip between tyrosine hydroxylase (TH)-immunoreactive and NADPH-d-reactive cell groups in normal human VLM and NTS, in view of their potential implications in human autonomic control and involvement in central autonomic disorders. Medullae from three patients with no neurologic disease were obtained at autopsy within 24 h of death. Individual sections, obtained from the rostral and caudal medulla, were stained for TH, NADPH-d or both. We found that: (1) TH-and NADPH-d positive neurons are topographically segregated in the VLM; (2) in the VLM, TH and NADPH-d neurons may coexist within a given area but both markers do not appear to coexist in single neuron; (3) NADPH-d-reactive fibers and processes overlap the distribution of TH neurons within the VLM; and (4) both TH-and NADPH-d-reactive processes appear to innervate intrinsic blood vessels in the VLM and NTS. Thus, there are important topographic relationships between catecholamine-and NO-synthesizing neurons in human VLM and perhaps NTS, including innervation of intrinsic blood vessels. This may have important implications in regulation of autonomic reflexes, sympathetic excitatory drive and intrinsic control of cerebral blood flow in humans.

AB - The human medulla contains catecholamine-and NADPH-diaphorase (NADPH-d) neurons in both the ventrolateral medulla (VLM) and nucleus of the solitary tract (NTS). There is abundant experimental evidence for the critical role of these areas in control of arterial pressure. We sought to determine the pattern of distribution and topographic relationhip between tyrosine hydroxylase (TH)-immunoreactive and NADPH-d-reactive cell groups in normal human VLM and NTS, in view of their potential implications in human autonomic control and involvement in central autonomic disorders. Medullae from three patients with no neurologic disease were obtained at autopsy within 24 h of death. Individual sections, obtained from the rostral and caudal medulla, were stained for TH, NADPH-d or both. We found that: (1) TH-and NADPH-d positive neurons are topographically segregated in the VLM; (2) in the VLM, TH and NADPH-d neurons may coexist within a given area but both markers do not appear to coexist in single neuron; (3) NADPH-d-reactive fibers and processes overlap the distribution of TH neurons within the VLM; and (4) both TH-and NADPH-d-reactive processes appear to innervate intrinsic blood vessels in the VLM and NTS. Thus, there are important topographic relationships between catecholamine-and NO-synthesizing neurons in human VLM and perhaps NTS, including innervation of intrinsic blood vessels. This may have important implications in regulation of autonomic reflexes, sympathetic excitatory drive and intrinsic control of cerebral blood flow in humans.

KW - Catecholamine

KW - Cerebral blood vessel

KW - Human medulla

KW - Nitric oxide

KW - Ventrolateral medulla

UR - http://www.scopus.com/inward/record.url?scp=0028999840&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028999840&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(95)00400-K

DO - 10.1016/0006-8993(95)00400-K

M3 - Article

C2 - 7583226

AN - SCOPUS:0028999840

VL - 684

SP - 215

EP - 220

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 2

ER -