Local proliferation and mobilization of CCR3+ CD34+ eosinophil-lineage-committed cells in the lung

Madeleine Rådinger, Apostolos Bossios, Margareta Sjöstrand, You Lu, Carina Malmhäll, Anna Karin Dahlborn, James J. Lee, Jan Lötvall

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Emerging evidence suggests that haematopoietic CD34+ progenitor cells migrate from bone marrow (BM) to sites of allergen exposure where they can undergo further proliferation and final maturation, potentially augmenting the degree of tissue inflammation. In the current study we used a well-characterized mouse model of allergen-induced airway inflammation to determine the role of CCR3 receptor-ligand interactions in the migration and function of CD34+ cells. Allergen exposure significantly increased BM, blood and airway CD34+ CCR3+ cells as well as airway CD34+ CCR3+ stem cell antigen-1-positive (Sca-1+) and CD34+ CD45+ interleukin-5 receptor-α-positive (IL-5Rα+) cells. A portion of the newly produced CD34+ CCR3+, Sca-1+ CCR3+ and IL-5Ralpha+ lung cells showed a significant proliferative capacity in response to allergen when compared with saline-treated animals. In addition, in vitro colony formation of lung CD34+ cells was increased by IL-5 or eotaxin-2 whereas eotaxin-2 had no effect on BM CD34+ cells. Furthermore, both eotaxin-1 and eotaxin-2 induced migration of BM and blood CD34+ CCR3+ cells in vitro. These data suggest that the CCR3/eotaxin pathway is involved in the regulation of allergen-driven in situ haematopoiesis and the accumulation/mobilization of eosinophil-lineage-committed progenitor cells in the lung. Hence, targeting both IL-5 and CCR3-mediated signalling pathways may be required to control the inflammation associated with allergen-induced asthma.

Original languageEnglish (US)
Pages (from-to)144-154
Number of pages11
JournalImmunology
Volume132
Issue number1
DOIs
StatePublished - Jan 2011

Fingerprint

Eosinophils
Allergens
Chemokine CCL24
Lung
Bone Marrow
Interleukin-5
Inflammation
CCR3 Receptors
Chemokine CCL11
Interleukin-5 Receptors
Stem Cells
Hematopoiesis
Hematopoietic Stem Cells
Interleukin-1
Bone Marrow Cells
Asthma
Ligands
Antigens

Keywords

  • Allergy
  • Cell proliferation
  • Eosinophils
  • Haematopoiesis
  • Inflammation
  • Lung

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Rådinger, M., Bossios, A., Sjöstrand, M., Lu, Y., Malmhäll, C., Dahlborn, A. K., ... Lötvall, J. (2011). Local proliferation and mobilization of CCR3+ CD34+ eosinophil-lineage-committed cells in the lung. Immunology, 132(1), 144-154. https://doi.org/10.1111/j.1365-2567.2010.03349.x

Local proliferation and mobilization of CCR3+ CD34+ eosinophil-lineage-committed cells in the lung. / Rådinger, Madeleine; Bossios, Apostolos; Sjöstrand, Margareta; Lu, You; Malmhäll, Carina; Dahlborn, Anna Karin; Lee, James J.; Lötvall, Jan.

In: Immunology, Vol. 132, No. 1, 01.2011, p. 144-154.

Research output: Contribution to journalArticle

Rådinger, M, Bossios, A, Sjöstrand, M, Lu, Y, Malmhäll, C, Dahlborn, AK, Lee, JJ & Lötvall, J 2011, 'Local proliferation and mobilization of CCR3+ CD34+ eosinophil-lineage-committed cells in the lung', Immunology, vol. 132, no. 1, pp. 144-154. https://doi.org/10.1111/j.1365-2567.2010.03349.x
Rådinger M, Bossios A, Sjöstrand M, Lu Y, Malmhäll C, Dahlborn AK et al. Local proliferation and mobilization of CCR3+ CD34+ eosinophil-lineage-committed cells in the lung. Immunology. 2011 Jan;132(1):144-154. https://doi.org/10.1111/j.1365-2567.2010.03349.x
Rådinger, Madeleine ; Bossios, Apostolos ; Sjöstrand, Margareta ; Lu, You ; Malmhäll, Carina ; Dahlborn, Anna Karin ; Lee, James J. ; Lötvall, Jan. / Local proliferation and mobilization of CCR3+ CD34+ eosinophil-lineage-committed cells in the lung. In: Immunology. 2011 ; Vol. 132, No. 1. pp. 144-154.
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